维A酸贴剂的体外透皮扩散研究

目的:研究自制的维A酸贴剂的体外透皮扩散率及在皮肤内的蓄积量。方法:按《中国药典》2005年版规定测定含量均匀度,以酸性异丙醇为溶剂,在波长352nm处测定维A酸,用透皮扩散仪测定透皮贴剂与市售维A酸乳膏对小鼠皮的扩散率及皮肤内的蓄积量。结果:贴剂的含量均匀度合格;测定的平均回收率为97.99%。测得含量为9.73mg/片,为标示量的97.3%;贴剂的扩散率虽低于乳膏,但皮肤内的蓄积量明显高于乳膏。结论:该贴剂能使药物蓄积于皮肤内,有利于提高对皮肤病的疗效和降低毒性,值得进一步开发。

Study on Transdermal Diffusion of Tretinoin Patches in vitro

Objective:To study the transdermal diffusion rate and the drug amount retained in skin of self-prepared tretinoin pat- ches in vitro.Method:Uniformity of drug content was determined according to Pharmacopoeia of China (2005);tretinoin was deter- mined by spectophotometrically at 352nm using acidic isopropyl alcohol as the solvent;and transdermal diffusion through mouse skin and drug residue on the skin were measured with tretinoin cream as the control in vitro.Result:Uniformity of drug content of the pat- ches was up to the standard.Mean recovery of the determination method was 97.99% and drug content was 9.73mg per patch (la- beled amount 97.3%).Transdermal diffusion rate of the patches was lower than that of the cream In vitro,however,the patches showed significantly higher drug amount retained in the skin.Conclusion:The assay method is reliable,and the patches show good ca- pacity to retain drug in the skin,which indicates the patches might enhance therapeutic efficiency against skin diseases and reduce ad- verse effects,thus are worthy of the study for further development.