| 培养角质形成细胞构成性表达热休克蛋白72 |
| |
| 引用本文： | 廖文俊,刘彦仿.培养角质形成细胞构成性表达热休克蛋白72[J].中国皮肤性病学杂志,1999,13(5):257-258. |
| |
| 作者姓名： | 廖文俊 刘彦仿 |
| |
| 作者单位： | [1]第四军医大学西京医院皮肤科 [2]第四军医大学病理学教研室 |
| |
| 摘 要： | 目的 了解热休克蛋白７２（ＨＳＰ７２）在培养角质形成细胞中的表达情况。方法 无血清培养基培养原代角质形成细胞，免疫组织化学（ＳＡＢＣ）及核酸原位杂交技术检测ＨＳＰ７２的表达。结果 角质形成细胞的细胞中常见阳性染色，少数大细胞中ＨＳＰ７２表达增强，集中在细胞核周围。结论：普通条件培养的皮肤角质形成细胞即可构成性表达ＨＳＰ７２，可能对维持角质形成细胞的正常功能和活性方面个有重要作用。
|
| 关 键 词： | 角质形成细胞 热休克蛋白 免疫组织化学 |
Constitutive HSP72 Expression of Keratinocytes in Culture. |
| |
| Liao Wenjun,Liu Yanfang,Liu Yufeng.Constitutive HSP72 Expression of Keratinocytes in Culture.[J].The Chinese Journal of Dermatovenereology,1999,13(5):257-258. |
| |
| Authors: | Liao Wenjun Liu Yanfang Liu Yufeng |
| |
| Institution: | Liao Wenjun,Liu Yanfang,Liu Yufeng.Department of Dermatology,Xijing Hospital,The Fourth Military Medical University,Xi'an,710032 |
| |
| Abstract: | Objective To investigate the expression of HSP72 in cultured keratinocytes.Methods Primary human skin keratinocytes were cultured in serum-free medium.The expression of HSP72 was detected with techniques of immunohistochemistry (Strepavidin-Biotin Complex) and in situ hybridization.Results Positive cytoplasmatic staining was observed in cultured keratinocytes,an increase of HSP72 expression which concentrated around the nuclei was also found in few large keratinocytes.Conclusion Keratinocytes cultured in common condition can constitutively express HSP72,which may play an important role in maintaining the normal function and activity of epidermal keratinocytes. |
| |
| Keywords: | Keratinocyte Heat shock protein Immunohistochemistry In situ hybridization |
| 本文献已被 CNKI 维普 等数据库收录！ |
