THE INHIBITORY EFFECT OF EXTRACT OF CAMELLIA SINENSIS AND EXTRACT OF CAMELLIA PTILOPHYLLA CHANG ON DNA POLYMERASE OF EHRLICH ASCITES CARCINOMA CELLS

ＤＮＡｐｏｌｙｍｅｒａｓｅ（Ｐｏｌ）ｉｓａｋｅｙｅｎｚｙｍｅｏｆＤＮＡｓｙｎｔｈｅｓｉｓ，ｉｔｃａｔａｌｙｚｅｓｄＮＴＰｐｏｌｙｍｅｒｉｚｅｄｉｎｔｏＤＮＡ，ｔｈｅ３’５’ｅｘｏｎｕｃｌｅａｓｅｆｕｎｃｔｉｏｎｏｆＰｏｌｅｎｓｕｒｅｓｔｈｅａｕｔｈ...

The inhibitory effect of extract of camellia sinensis and extract of Camellia Ptilophylla Chang on dna polymerase of ehrlich ascites carcinoma cells

Objective: To detect the effect of extract of Camellia Sinensis (ECS) and extract of Camellia Ptilophylla Chang (ECPC) on DNA polymerase (Pol) of Ehrlich ascites tumor cells. Methods: Referring to the method of K.Ono, Pol was extracted from Ehrlich ascites tumor cells in mice. Pol α, β, and γ were separated by phosphocellulose column chromatography and were identified. The effect of ECPC and ECS on Pol was studied. Results: ECPC and ECS were shown to inhibit the activity of Pol α, β, and γ. IC₅₀ values of ECS on Pol α, β, and γ were 10.2 μ g/ml, 9.9 μ g/ml and 28.9 μ g/ml respectively. IC₅₀ values of ECPC on Pol α, Pol β and Pol γ were 5.6 μ g/ml, 15 μ g/ml and 14.7 μ g/ml respectively. The modes of inhibition of ECPC on Pol α, Pol β and Pol γ were noncompetitive with respect to template DNA. The Ki values of ECPC on Pol α, β, and γ were 2.68±0.12 μ g/ml, 2.24±0.12 μ g/ml, 2.56±0.18 μ g/ml. Conclusion: ECPC and ECS were shown to have inhibitory effect on DNA polymerase of tumor cells. The mode of inhibition of ECPC on Pol α, Pol β and Pol γ were noncompetitive with respect to template DNA. Project supported by The National Natural Science Foundation of China, No.39070918