慢性乙型肝炎病人血液单核细胞中HBV标志物的检出

本文报告在慢性乙型肝炎38例的外周血单核细胞(PBMC)和T、B淋巴细胞中检测到HBV标志物阳性29例(76.3％)。其中17例病人的PBMC中HBsAg,HBeAg和HBV-DNA阳性者分别为4,2和14例。21例病人分离出T,B淋巴细胞,其中T细胞中HBsAg,HBeAg与HBV-DNA阳性分别为2,2和1例;B细胞中分别为3,6,1例。血清HBV标志物阳性的病人其PBMC中这些标志物的检出率(13/15例)明显高于血清抗HBc和抗HBe阳性的稳定期病人(3/7例)。提示HBV可能感染和损害慢性乙型肝炎病人的淋巴细胞,这也许是乙型肝炎病人免疫功能异常的原因之一。

DETECTION OF HBV MARKS IN PERIPHERAL BLOOD MONONUCLEAR CELLS FROM CHRONIC HEPATITIS

In this study, we detected HBV marks in the peripheral blood mononuclear cells (PBMCs) and T, B lymphocytes from 38 patients with chronic hepatitis B.HBV marks were found in 29/38 cases (76.3%).Out of 17 patients 4 have positive HBsAg result, 2 positive HBeAg and 14 positive HBV-DNA in PBMCs. Positive HBsAg and HBeAg were found in T cells in 2 out of 21 patients, and HBV-DNA, in 1 patient.Positive HBsAg was observed in B cells in 3 of 21 patients, positive HBeAg in 6, positive HBV-DNA in 1.The positive ratios of HBV marks of active patients were higher than those of stable ones.These results suggest that HBV may destroy lymphocytes directly, especially during B the active stage of hepatitis,which may be one of the causes for immunological abnormality in chronic hepatitis B patients.