Panax notoginseng saponins inhibit ischemia-induced apoptosis by activating PI3K/Akt pathway in cardiomyocytes |
| |
Authors: | Chen Shaoxian Liu Juli Liu Xiaoying Fu Yongheng Zhang Mengzhen Lin Qiuxiong Zhu Jiening Mai Liping Shan Zhixin Yu Xiyong Yang Min Lin Shuguang |
| |
Institution: | a Medical Research Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China b Medical College of Shantou University, Shantou 515041, China c Department of Pharmacy, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China d Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China |
| |
Abstract: | Aim of this studyThe panax notoginseng saponins (PNS) have been clinically used for the treatment of cardiovascular diseases and stroke in China. Evidences demonstrated that PNS could protect cardiomyocytes from injury induced by ischemia, but the underlying molecular mechanisms of this protective effect are still unclear. This study was aimed to investigate the protective effect and potential molecular mechanisms of PNS on apoptosis in H9c2 cells in vitro and rat myocardial ischemia injury model in vivo.Materials and methodsH9c2 cells subjected to serum, glucose and oxygen deprivation (SGOD) were used as in vitro models and SD rats subjected to left anterior descending (LAD) coronary artery ligation were used as in vivo models. The anti-apoptotic effect of PNS was evaluated by Annexin V/PI analysis or TUNEL assay. Mitochondrial membrane potential (Δψm) was detected by JC-1 analysis. The expression of Akt and phosphorylated Akt (p-Akt) were detected by western blot assay.ResultsPNS exhibited anti-apoptotic effect both in H9c2 cells and in ischemic myocardial tissues. However, the effect was blocked in vitro by LY294002, a specific PI3K inhibitor. The anti-apoptotic effect of PNS was mediated by stabilizing Δψm in H9c2 cells. Furthermore the indices of the left ventricular ejection fractions (EF), left ventricular fractional shortening (FS), left ventricular dimensions at end diastole (LVDd) and left ventricular dimensions at end systole (LVDs) suggested that PNS improved rats cardiac function. PNS significantly increased p-Akt both in H9c2 cells and in ischemic myocardial tissues and this effect was also blocked by LY294002 in H9c2 cells.ConclusionResults of this study suggested that PNS could protect myocardial cells from apoptosis induced by ischemia in both the in vitro and in vivo models through activating PI3K/Akt signaling pathway. |
| |
Keywords: | PNS panax notoginseng saponins SGOD serum glucose and oxygen deprivation EF left ventricular ejection fractions FS left ventricular fractional shortening LVDd left ventricular dimensions at end diastole LVDs left ventricular dimensions at end systole TUNEL terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|