Antinociceptive effect of the essential oil of Zingiber zerumbet in mice: possible mechanisms |
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Authors: | Khalid Mohamed Hanief Akhtar Muhammad Nadeem Mohamad Azam Shah Perimal Enoch Kumar Akira Ahmad Israf Daud Ahmad Lajis Nordin Sulaiman Mohd Roslan |
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Affiliation: | a Department of Human Anatomy, Universiti Putra Malaysia, Serdang, Malaysia b Department of Biomedical Science, Universiti Putra Malaysia, Serdang, Malaysia c Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia d Department of Chemistry, Universiti Putra Malaysia, Serdang, Malaysia |
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Abstract: | Ethnopharmacological relevanceZingiber zerumbet (L.) Smith, a wild edible ginger species or locally known as “lempoyang”, commonly used in the Malays traditional medicine as an appetizer or to treat stomachache, toothache, muscle sprain and as a cure for swelling sores and cuts.AimThe present study was conducted to investigate the possible mechanism of actions underlying the systemic antinociception activity of the essential oil of Zingiber zerumbet (EOZZ) in chemical-induced nociception tests in mice.Materials and methodsAcetic acid-induced abdominal constriction, capsaicin-, glutamate- and phorbol 12-myristate 13-acetate-induced paw licking tests in mice were employed in the study. In all experiments, EOZZ was administered systemically at the doses of 50, 100, 200 and 300 mg/kg.ResultsIt was shown that EOZZ given to mice via intraperitoneal and oral routes at 50, 100, 200 and 300 mg/kg produced significant dose dependent antinociception when assessed using acetic acid-induced abdominal writing test with calculated mean ID50 values of 88.84 mg/kg (80.88-97.57 mg/kg) and 118.8 mg/kg (102.5-137.8 mg/kg), respectively. Likewise, intraperitoneal administration of EOZZ at similar doses produced significant dose dependent inhibition of neurogenic pain induced by intraplantar injection of capsaicin (1.6 μg/paw), glutamate (10 μmol/paw) and phorbol 12-myristate 13-acetate (1.6 μg/paw) with calculated mean ID50 of 128.8 mg/kg (118.6-139.9 mg/kg), 124.8 mg/kg (111.4-139.7 mg/kg) and 40.29 (35.39-45.86) mg/kg, respectively. It was also demonstrated that pretreatment with l-arginine (100 mg/kg, i.p.), a nitric oxide precursor significantly reversed antinociception produced by EOZZ suggesting the involvement of l-arginine/nitric oxide pathway. In addition, methylene blue (20 mg/kg, i.p.) significantly enhanced antinociception produced by EOZZ. Administration of glibenclamide (10 mg/kg, i.p.), an ATP-sensitive K+ channel antagonist significantly reversed antinociceptive activity induced by EOZZ.ConclusionTogether, the present results suggested that EOZZ-induced antinociceptive activity was possibly related to its ability to inhibit glutamatergic system, TRPV1 receptors as well as through activation of l-arginine/nitric oxide/cGMP/protein kinase C/ATP-sensitive K+ channel pathway. |
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Keywords: | Zingiber zerumbet (L.) Smith Essential oil Zingiberaceae Antinociceptive Nitric oxide Cyclic GMP Potassium channels |
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