TK1、Ki-67在非小细胞肺癌组织中的表达及预后意义

目的:探讨细胞质胸苷激酶1(thymidine kinase 1,TK1)和Ki-67在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达水平及其对NSCLC患者预后的意义?方法:将202例NSCLC及63例癌旁肺组织标本制作成组织芯片,采用免疫组织化学EnVision法检测TK1和Ki-67的表达,探讨其表达水平与临床病理特征及预后的关系?结果:NSCLC组织中TK1(60.89%)和Ki-67(55.45%)的阳性表达率均显著高于癌旁肺组织(P < 0.01);Spearman等级相关分析显示二者的表达呈正相关(r = 0.518, P < 0.001);NSCLC中,TK1和Ki-67的表达均与肿瘤分化程度和TNM分期显著相关(P < 0.05);Kaplan-Meier生存分析显示随TK1表达的增强和Ki-67指数的增高,NSCLC患者的总体生存期逐渐降低(P < 0.01);Cox 回归分析提示肿瘤TK1表达和TNM分期是影响NSCLC患者预后的独立危险因素(P < 0.05)?结论:TK1在NSCLC的表达与肿瘤的分化?进展有关,TK1表达有助于对患者预后的评价?

The expression and prognostic significance of TK1 and Ki-67 in non-small cell lung cancer

Objective:To investigate the expressions of thymidine kinase 1(TK1) and Ki-67 in non-small cell lung cancer(NSCLC)and their prognostic value in NSCLC. Methods:The tissue microarrays which contain 202 NSCLC cases and 63 normal lung cases were constructed. The expressions of TK1 and Ki-67 were detected in these tissues by immunohistochemistry method. The relationship between expression levels of two makers and the clinicopathological and survival parameters of NSCLC were evaluated. Results:The positive expression rates of TK1(60.89%)and Ki-67(55.45%)in the NSCLC tissues were significantly higher than those in normal tissue group (P < 0.000 1,P < 0.000 5). The Spearman correlation analysis showed that the expression of TK1 were positively correlated with Ki-67 expression (r = 0.518,P < 0.001). In the group of NSCLC, both TK1 and Ki-67 expression were significant correlated with tumor grade and TNM stage. Kaplan-Meier survival analysis indicated that the survival rate was markedly lower in patients with high expression level of two markers than those with low expression level (P < 0.05);Multivariate analysis using Cox regression model identified that TNM stage and TK1 expression were independent prognostic factors of NSCLC (P < 0.05). Conclusion:The expression of TK1 was significantly correlated with tumor grade and TNM stage. TK1 might be employed to evaluate the prognosis of NSCLC patients.