氟伐他汀对高糖腹透液诱导人腹膜间皮细胞纤维连接蛋白表达的影响

目的:探讨氟伐他汀(fluvastatin,Flu)对高糖腹透液(high-glucose peritoneal dialysate,HGPDS)诱导人腹膜间皮细胞(human peritoneal mesothelial cells,HPMCs)纤维连接蛋白(fibronectin,FN)表达的影响?方法:体外培养HPMCs,同步化24 h后,分为正常对照组?HGPDS组?HGPDS+Flu组?单纯氟伐他汀组?DMSO对照组,各组分别刺激不同时间后,噻唑蓝(MTT)检测各组细胞的活力,RT-PCR检测FN的mRNA表达,ELISA法检测上清液FN蛋白表达的变化?结果:与正常对照组比较,HGPDS明显抑制细胞活力,HGPDS联合Flu共同培养24?36 h,细胞活力有所恢复,其中,1 × 10-6 mol/L Flu作用24 h,细胞活力改善差异有统计学意义(P < 0.05);与正常对照组比较,HGPDS明显增加人腹膜间皮细胞FN mRNA及蛋白表达(P < 0.05),并呈时间依赖性,其中FN mRNA于6 h达高峰,FN蛋白于24 h达高峰?Flu 可抑制HGPDS诱导的FN的高表达(P < 0.05),并呈浓度依赖性?结论:HGPDS诱导体外培养人腹膜间皮细胞FN表达增加,此作用可被Flu 抑制?

The effect of fluvastatin on the expression of fibronectin in human peritoneal mesothelial cells induced by high-glucose peritoneal dialysate

Objective:To explore the effect of fluvastatin(Flu) on the expression of fibronectin(FN) in human peritoneal mesothelial cells (HPMCs) induced by high-glucose peritoneal dialysate (HGPDS). Methods:After incubated in DMEM with 0.01%FBS for 24 h,cultured HPMCs were randomly divided into control group,HGPDS group,HGPDS+Flu group,Flu alone group and DMSO control group. HPMCs were treated for 3,6,12,24,36,48,72 h respectively and the cellular viability were detected by MTT colorimetry. The mRNA expression of FN was observed by RT-PCR. HPMCs were treated for 24 h and the levels of FN in the culture media were measured by ELISA. Results:The results of MTT revealed that lower viability was found in the cells treated with HGPDS and partialy restored in cells treated with different concentrations of Flu at 24 h and 36 h when compared with control group. The significant improvement of cell viability was shown in Flu treatment at concentration of 1 × 10-6 mol/L at the time of 24 h(P < 0.05). Compared with the normal control group,the mRNA and protein expressions of FN increased time-dependently in HPMCs treated with HGPDS(P < 0.05),which peaked at 6 h and 24 h respectively. The fluvastatin significantly inhibited the effects of HGPDS on the expression of FN in a dose-dependent manner(P < 0.05). Conclusion:HGPDS can increase FN expression in HPMCs,which will be inhibited by Flu.