血清差异表达多肽谱用于人卵巢癌诊断的研究

目的:建立卵巢癌患者血清差异表达多肽谱,并探讨其潜在诊断价值?方法:应用磁珠富集多肽结合基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF-MS)检测方法,筛选上皮性卵巢癌组和健康对照组差异表达多肽峰,利用ClinProTools软件比较两组血清多肽表达差异并建立差异表达多肽谱及早期诊断模型?结果:在卵巢癌组血清中,m/z 2 662.02?2 653.68?5 859.21?5 901.69?5 937.06表达显著上调;m/z 1 857.82?2 074.28?2 856.33表达显著下调?差异表达图谱建立的诊断模型预测早期卵巢癌的准确率达到81.8%(9/11),利用9例宫颈癌血清验证均为阴性,特异性达100%(0/9)?结论:磁珠富集-质谱结合ClinProTools生物信息学分析技术,有望成为新的人卵巢癌辅助诊断技术?

Mass spectrometry analysis of sera peptides in diagnosis of ovarian cancer

Objective:To establish the expression profiles of peptides in sera of ovarian cancer and explore the early-diagnosis value of expression profiles of peptides in ovarian cancer. Methods:Magnetic binding-weak cation exchange beads (MB-WCX) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis were used to detect expression files of peptides. The early-diagnosis pattern was established by differential peptides peaks using the ClinProTool data analysis program. Results:In the mass range from 1 000 to 15 000 Da,8 peaks with clear differences in amplitude between the patients and health controls were detected,5 peaks with mass charge ratio(m/z) of 2 662.02, 2 653.68,5 859.21,5 901.69 and 5 937.06 were up-regulated notably,and 3 peaks with m/z of 1 857.82,2 074.28 and 2 856.33 were markedly down-regulated in patients with ovarian cancer. In specimens of early-stage ovarian cancer,the accuracy of characteristic proteomic patterns detection could be 81.8%(9/11),and the specificity showed 100%. Conclusion:MB-WCX separation coupled with MALDI-TOF-MS and ClinProTools analysis could be a desired method to find bio-markers in ovarian cancer,and could have a potential value in the early diagnosis of ovarian cancer.