用DIFA 检测肿瘤患者的弓形虫抗体

目的: 为了解湖北肿瘤患者弓形虫感染概况并探索新技术直接免疫固相凝集试验(DIFA)等3项试验联合筛选诊断弓形虫感染的意义。方法: 使用DIFA检测100 例肿瘤患者的弓形虫IgG 抗体, 阳性者用IgG-IFAT 复查, 再用Toxo-IgG/IgA/IgM-ISAGA 检测。结果: DIFA 检测阳性率为12% (12/100), IgG-IFAT 检测符合率为100%; IgA 可疑1 例; IgM 抗体阳性4 例, 可疑1 例, 其中2 例提示为活动性感染。合并弓形虫感染的12 例分布于肺癌、非何杰金氏淋巴瘤和乳腺癌患者中, 其感染率分别为受检人数的22.2% (6/27)、19.3% (5/26) 及7.1% (1/14)。结论: DIFA 的敏感性和特异性均较佳, 3 种方法的联合应用适宜作弓形虫感染的常规筛选诊断。提示免疫功能降低的肿瘤患者, 特别是肺癌和非何杰金氏淋巴瘤患者容易感染弓形虫病或促使潜伏的弓形虫病活动。

DETECTION OF ANTI-TOXOPLASMA ANTIBODIES FROM TUMOR PATIENTS BY DIFA

AIM:To survey the prevalence of toxoplasmosis in tumor patients in Hubei and to ex- amine the significance of direct immunofixation agglutination(DIFA) ,Ig G- IFAT,and IS- AGA for dignosing Toxoplasma infection.METHODS:Sera from1 0 0 tumor cases were test- ed by DIFA for detecting anti- Toxoplasma Ig G antibodies.The positive sera were re- exam- ined by Ig G- IFAT and then detected by Toxo- Ig G/ Ig A/ Ig M- ISAGA.RESUL TS:Using DI- FA,the total anti- Toxoplasma antibody positive rate was 1 2 % (1 2 / 1 0 0 ) .Using Ig G- IFAT, the corresponding rate was1 0 0 % ,with one case suspected to be Ig A positive.Four cases were Ig M positive,two of them were with active infection and one was dubious.The rates of Toxoplasma infection in patients with lung cancer,non- Hodgkin lymphoma and breastcancer were2 2 .2 % (6 / 2 7) ,1 9.3% (5 / 2 6 ) and7.1 % (1 / 1 4) ,respectively.CONCL USION:DIFA is more sensitive and specific for diagnosing Toxoplasma infection and that cancer patients are more susceptible to Toxoplasma infection.