顺式氯氰菊酯对嗜人按蚊和斯氏按蚊幼虫蛋白含量、蛋白酶和羧酸酯活性的影响

目的 :探讨杀虫剂顺式氯氰菊酯 (奋斗呐 )灭蚊作用机理。方法 :分别用 0 .697nmol/ L、1.393nmol/ L奋斗呐处理嗜人按蚊和斯氏按蚊 3龄幼虫 ,待生长至 4龄 ,测定其蛋白质含量及蛋白酶和羧酸酯酶活力。结果 :与对照组相比 ,用药组幼虫蛋白质含量显著增加 ,蛋白酶与羧酸酯酶活力显著降低 ,具有非常显著性差异 ( P<0 .0 1)。结论 :本研究结果表明奋斗呐对蚊幼虫蛋白质含量及蛋白酶和羧酸酯酶活性均有显著的影响。

EFFECT OF ALPHAMETHRIN ON PROTEIN CONTENT, PROTEINASE AND CARBOXYLESTERASE ACTIVITIESIN LARVAE OF ANOPHELES ANTHROPOPHAGUS AND ANOPHELESSTEPHENSI

AIM:To study the mechanism of the cidal effect of alphamethrin (fendona) against mosquitoes.METHODS:The third instar larvae of Anopheles anthropophagus and Anopheles stephensi were treated with0 .6 97nmol/L and1 .393nmol/L of fendona,respectively.The fourth instar larvae were collected.The protein content,proteinase and carboxylesterase ac- tivities in the fourth instar larvae were determined by spectrophotometric methods.RESULTS:When compared with the control groups,the protein content was increased and the specific activities of proteinase and carboxylesterase were reduced in the treatcd groups, the difference being markedly significant (P < 0. 01). CONCLUSION: The result showed that fendona remarkably influenced the protein content and activities of proteinase and carboxylesterase in the larvae of An. anthropophagus and An. stephensi.