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日本血吸虫 NADH脱氢酶 1和细胞色素 C氧化酶 1基因部分序列
引用本文:邱持平,张立华,E.Sorensen,D.P.McManus. 日本血吸虫 NADH脱氢酶 1和细胞色素 C氧化酶 1基因部分序列[J]. 中国寄生虫学与寄生虫病杂志, 1997, 15(6): 378-381
作者姓名:邱持平  张立华  E.Sorensen  D.P.McManus
作者单位:1 中国预防医学科学院寄生虫病研究所; 2 Molecular Helminthology Laboratory, Tropical Health Program, The Queensland Institute of Medical Research, Australia
摘    要:目的 :测定和比较日本血吸虫浙江株和安徽株 NADH脱氢酶 1( NADH1)和细胞色素 C氧化酶 1( Cytc1)基因部分序列。方法:用 PCR方法从特定的引物扩增出 NADH1和 Cytc1DNA基因 ,然后进行测序。结果 :获得两株日本血吸虫成虫 NADH1和 Cytc1基因及其序列。结论 :两株日本血吸虫成虫 NADH1基因及其部分序列除第 382位核苷酸不同 ( C/T)外 ,其余的均相同。两株日本血吸虫成虫Cytc1基因及其部分序列完全相同。

关 键 词:日本血吸虫  NADH脱氢酶1  细胞色素C氧化酶1  PCR  DNA序列

PARTIAL SEQUENCE OF NADH DEHYDROGENASE 1 AND CYTOCHROME C OXIDASE 1 GENE OF SCHISTOSOMA JAPONICUM
Qiu Chiping,Zhang Lihua,E.Sorensen,D.P.Mc Manus. PARTIAL SEQUENCE OF NADH DEHYDROGENASE 1 AND CYTOCHROME C OXIDASE 1 GENE OF SCHISTOSOMA JAPONICUM[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1997, 15(6): 378-381
Authors:Qiu Chiping  Zhang Lihua  E.Sorensen  D.P.Mc Manus
Affiliation:1 Institute of Parasitic Diseases, Chinese Academy of Prevantive Medicine, Shanghai 200025 2 Molecular Helminthology Laboratory, Tropical Health Program, The Queensland Institute of Medical Research, Australia
Abstract:AIM:To determine and compare NADH dehydrogenase1( NADH1) and cytochrome C oxidase1( Cytc1) partial gene sequences of Zhejiang and Anhui isolates of Schistosoma japonicum. METHODS: NADH1 and Cytc1 genes were amplified by PCR using special primers corresponding with 44 1and 370 bp of the genomic DNAs, and subsequently sequenced. RESULTS:NADH1 and Cytc1 gene sequences were obtained from the two isolates. CONCLUSION: The partial sequences of the NADH1 genes were essentially identical for both isolates with only one nucleotide difference (C/T ) at position 382. The Cytc1 sequences were virtually identical for the two isolates.
Keywords:Schistosom a japonicum  NADH dehydrogenase1  cytochrome C oxidase1  PCR  sequence
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