| 日本血吸虫大陆株副肌球蛋白部分基因克隆 |
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| 引用本文： | 邱持平,童小妹.日本血吸虫大陆株副肌球蛋白部分基因克隆[J].中国寄生虫学与寄生虫病杂志,1997,15(2):104-106. |
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| 作者姓名： | 邱持平 童小妹 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所!上海200025 |
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| 基金项目： | 国家 8 6 3高科技基金 |
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| 摘 要： | 目的 :克隆日本血吸虫大陆株副肌球蛋白部分基因。方法 :用 PCR方法根据已发表日本血吸虫菲律宾株副肌球蛋白基因部分核苷酸序列扩增大陆株副肌球蛋白基因。结果 :获得一日本血吸虫大陆株 72 0 bp基因克隆。结论 :经核苷酸序列分析证实 ,本克隆基因与菲律宾株基因核苷酸同源性为 99.0 3%。
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| 关 键 词： | 日本血吸虫 副肌球蛋白 基因克隆 疫苗 PCR 核苷酸序列 |
CLONING OF A PARTIAL PARAMYOSIN GENE FROM SCHISTOSOMA JAPONICUM( CHINESE MAINLAND ORIGIN) |
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| Qiu Chiping,Tong Xiaomei,Liu Shuxian Institute of Parasitic Diseases,Chinese Academy of Preventive Medicine,Shanghai.CLONING OF A PARTIAL PARAMYOSIN GENE FROM SCHISTOSOMA JAPONICUM( CHINESE MAINLAND ORIGIN)[J].Chinese Journal of Parasitology and Parasitic Diseases,1997,15(2):104-106. |
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| Authors: | Qiu Chiping Tong Xiaomei Liu Shuxian Institute of Parasitic Diseases Chinese Academy of Preventive Medicine Shanghai |
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| Institution: | Qiu Chiping,Tong Xiaomei,Liu Shuxian Institute of Parasitic Diseases*,Chinese Academy of Preventive Medicine,Shanghai2 0 0 0 2 5 |
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| Abstract: | AIM:To clone a partial paramyosin gene from Schistosoma japonicum of Chinese main- land origin.METHODS:The gene was amplified by PCR according to the published partial paramyosin sequence of S.japonicum of Philippine origin.RESULTS:A gene of about72 0 bp was cloned from S.japonicum of Chinese mainland origin.CONCL USION:The analysis of the sequence of this gene revealed 99.0 3% identity with thatof S.japonicum of Philippine origin. |
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| Keywords: | Schistosom a japonicum paramyosin gene cloning PCR vaccine sequenc |
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