蒿甲醚体外抗弓形虫作用的实验研究

目的 :了解蒿甲醚体外抗弓形虫速殖子的作用效果。方法 :采用大鼠肾细胞培养技术 ,观察蒿甲醚 (浓度 0.01- 100 μg/ ml)对虫体侵入细胞及在细胞内增殖的影响 ,选乙酰螺旋霉素作对照。结果 :蒿甲醚抗弓形虫作用的最低有效浓度为 0 .1μg/ml。当其浓度为 4 μg/ ml时 ,作用速殖子 4 8h后 ,光镜下多数虫体变形 ,胞质内出现空泡、颗粒。乙酰螺旋霉素的作用不及相同浓度的蒿甲醚。结论 :蒿甲醚在体外能抑制弓形虫速殖子的侵袭和繁殖力 ,且具有杀虫效果。

EXPERIMENTAL STUDY ON THE EFFECT OF ARTEMETHER AGAINST TOXOPLASMA

AIM:To study the activities of artemether(Art) against the tachyzoite of Toxoplasma gondii in vitro. METHODS:The method of cell culture of rat kidney was used for investigation the effects of art (concentration0.0 1-100 μg/ml) on the invasion and proliferation of T.gondii. Acetylspiramycin was used as control. RESULTS:The minimum effective concentration of Artagainst T.gondii was 0.1 μg/ml. When the concentration of Art was 4μg/ml, most of the Toxoplasma organisms appeared deformed and distorted after 48h with vacuoles and granules in its cytoplasm under light microscope. The anti Toxoplasma effect of acetylspiramycin was inferior to that of Art at the same concentration. CONCLUSION: Art could inhibit the invasion and multiplication of T.gondii tachyzoites and kill them in vitro. The toxoplasm acidal effect of Art was proved to be superior to that of acetylspiramycin.