| TPOAb液相竞争放免分析法的建立及临床应用 |
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| 引用本文： | 唐金凤,王铸钢,陈春荣,李凤英,许曼音,陈家伦.TPOAb液相竞争放免分析法的建立及临床应用[J].放射免疫学杂志,1997(6). |
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| 作者姓名： | 唐金凤 王铸钢 陈春荣 李凤英 许曼音 陈家伦 |
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| 作者单位： | 上海第二医科大学附属瑞金医院 上海市内分泌研究所200025 |
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| 摘 要： | 本文应用人甲状腺过氧化物酶(hTPO)单克隆抗体和hTPO单抗免疫亲和层析纯化的hTPO,用Iodogen法标记,建立了人甲状腺过氧物酶液相竞争的放射免疫测定。研究结果表明,本法灵敏度高,特异性强,重复性好,可取代MCA的测定,作为自身免疫性甲状腺疾病(AITD)尤其是桥本氏甲状腺炎诊断和疗效判断的重要指标。
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| 关 键 词： | 甲状腺疾病 甲状腺过氧化物酶抗体 放射免疫分析 |
DEVELOPMENT OF A METHOD OF LIQUID COMPETITIVE RIA FOR TPOAb AND ITS CLINICAL USAGE |
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| Tang Jin-feng,Wang Zhu-gang,Chen Chun-rong,et al..DEVELOPMENT OF A METHOD OF LIQUID COMPETITIVE RIA FOR TPOAb AND ITS CLINICAL USAGE[J].Journal of Radioimmanology,1997(6). |
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| Authors: | Tang Jin-feng Wang Zhu-gang Chen Chun-rong |
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| Institution: | Tang Jin-feng,Wang Zhu-gang,Chen Chun-rong,et al.Shanghai Research Institute of Endocrinology,Rui-Jin Hospital,Shanghai Second Medical University,200025 |
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| Abstract: | Using human thyroid peroxidase (hTPO) monoclonal antibody (mAb) to purify hTPO through affinitive chromatography we labelled the hTPO with 125J by Iodogen and developed a method of liquid competitive RIA for TPOAb testing. The results showed that the method had high sensitivity, specificity and good reproducibility and it might replace MCA test for diagnosis and: clinical follow-up in autoimmune thyroid disease, especially Hashimoto thyroiditis. |
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| Keywords: | Thyroid disease Thyroid peroxidase antibody Radioimmunoassay |
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