参附注射液对肠缺血-再灌注大鼠肺损伤的影响

目的探讨参附注射液（SF）对肠缺血-再灌注（ⅡR）所致肺损伤的防治作用及其对肿瘤坏死因子-α（TNF-α）、诱生型一氧化氮合酶（iNOS）和细胞间黏附分子-Ⅰ（ⅠCAM-1）的影响,及其防治肠源性肺损伤机制。方法SD大鼠随机分为缺血再灌注组、假手术组、SF组并予相应处理。以病理学改变作为评价肠、肺损伤的指标;监测再灌注前后平均动脉压变化;ELISA法检测血浆、肺组织TNF-α含量;用免疫组织化学方法检测肺及小肠组织iNOS和ICAM-1蛋白的表达。结果SF可明显减轻IIR导致的肺和小肠组织的病理损害,抑制IIR后出现的血浆及肺组织TNF-α水平升高,肺及小肠组织iNOS和ICAM-1蛋白表达的增加;再灌注后SF组平均动脉压变化较缺血再灌注组明显减轻。结论SF有防止肠源性肺损伤的发生,进而预防组织病程向多器官功能不全综合征发展的重要作用,这种作用可能通过抑制TNF-α、ⅠCAM-1和iNOS等介质的释放而实现。

Effects of Shenfu Injection on Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats

Objective： To explore the mechanism of Shenfu Injection（SF）＇ s protective effect on lung injury induced by intestinal ischemia- reperfusion（IIR） in rats. Methods： Adult Sprague- Dawley rats were divided into three groups： IR group, sham group and SF group, Pathologic methods were used to evaluate the degree of lung and small intestine injury. Plasma and pulmonary tumor necrosis factor-α（TNF -α） levels in each group were measured by ELISA, in addition, the expression levels and distribution of inducible nitric oxide synthase（iNOS） and intercellular adhesion molecule- I（ICAM- 1） in small intestine and lung tissues were determined by immuno- histochemical method. ResuLts： SF could significantly alleviate the pathologic lesion of lung and small intestine and improve the hypotensive status caused by IIR. It could also decrease the elevation of plasma and pulmonary TNF - α levels after IIR. Meanwhile, SF could markedly decrease the high expression levels of iNOS and ICAM - 1 after IIR. Conclusion： The result indicate that pretreatment with SF can prevent lung injury following IIR in rats, and such protective effect may be related to the inhibition of TNF - α, iNOS and ICAM - 1 expression.