人胎盘间充质干细胞的生物学特性及超微结构

目的 研究胎盘间充质干细胞生物学特性和超微结构,并探讨其在骨组织工程学中修复重建的应用前景.方法 胎盘间充质干细胞,测定其细胞周期及凋亡率情况,原子力显微镜观察细胞的表面结构,及向成骨细胞诱导分化.结果 人胎盘间充质干细胞为成纤维细胞样,增殖能力强,9.7％的细胞处于G2/M期或者S期.超微结构分析显示其细胞代谢活跃,黏附能力强,能被诱导分化为成骨细胞.结论 人胎盘间充质干细胞具备适合应用于骨组织工程学的良好生物学特性,并避免了免疫排斥和伦理问题,可望成为骨组织修复重建中的一个新的治疗途径.

Biological characteristics and ultrastructure of human placenta-derived mesenchymal stem cells

Objective To study the biological characteristics and cell ultrastructure of placentaderived mesenchymal stem cells (PDMSCs),and to evaluate their potential use for repair and reconstruction in bone tissue engineering.Methods PDMSCs were isolated and cultured from healthy human placenta.then were determined for cell cycle and apoptotic rate.Cell surface topography was evaluated under atomic force microscopy.PDMSCs were for osteoblastic differentiation.Results Human placenta- derived mesenchymal stem cells were fibroblast-like and active proliferating cells.9.7％ PDMSCs was in G2/M or S phase.Ultrastructural analysis showed active metabolism,significant adhering and successful osteoblastic differentiation of the cells.Conclusions PDMSCs have favorable biological properties for use in bone tissue engineering.The use of human placenta-derived mesenchymal stem cells may circumvent immune rejection and ethic problems,and play an essential role in the clinical treatment for large-size bone defects.