紫杉醇对膀胱癌EJ细胞株增殖抑制及诱导凋亡的作用

目的:探讨紫杉醇体外抑制膀胱癌E J细胞株及诱导凋亡的作用。方法:体外培养膀胱癌E J细胞株,以不同浓度紫杉醇处理12~72h后,通过M TT法测定细胞生长抑制作用,采用电镜观察和DNA琼脂糖凝胶电泳检测细胞凋亡。结果:紫杉醇能抑制E J细胞的生长,并在一定剂量和时间范围内呈现出明显的时间、浓度依赖关系。电镜下凋亡细胞体积缩小,染色质浓缩致密,并沿核膜分布形成花瓣形。电泳见出现典型的凋亡DNA梯形带。结论:紫杉醇能抑制膀胱癌E J细胞株的生长,诱导凋亡可能为其抗肿瘤作用机制之一。

Growth Inhibition and Apoptosis Induction of Bladder Cancer EJ Cells Line by Paclitaxel

Objective: To investigate the effect of inhibition and apoptosis induction of paclitaxel on bladder Cancer EJ cells line in vitro.Methods: Bladder Cancer EJ cells line cultured in vitro were treated by paclitaxel with different concentration for 12h-72h.Methyl thiazolyl tetrazolium(MTT) assay was used to measure the effect of the growth inhibition.Apoptosis of the cells were observed by electron microscopy and agarose gel electrophoresis.Results: Paclitaxel could inhibit the growth of the cells and present the dependent relationship of time and concentration in the range of time and dose.Volume of the apoptosis cells reduce,chromatin Concentrate and show petal form along nuclei membrane by electron micrscopy.DNA of the apoptosis cells display the ladder tapes.Conclusions:It may be one of the anticarcinogenic mechanism that paclitaxel could inhibit the growth of the bladder cancer EJ cells and induce cells apoptosis.