Immunoaffinity columns and determination of ochratoxin A in cereals by HPLC. Part II. Evaluation of extraction using acetonitrile/water

Ochratoxin A from wheat and rye grain was extracted with acetonitrile:water (60:40). After clean-up of extracts using immunoaffinity columns (IAC), ochratoxin was determined by high-performance liquid chromatography using C18 column and fluorometric detection at 330 nm excitation and 460 nm emission. The mean recovery of ochratoxin A at fortification levels 0.1-100 microg/kg, was 74-89%. The limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.025 microg/kg, respectively. The positive results were confirmed by reaction with BF3 complex in methanol.