苯那普利后处理对离体大鼠心肌缺血再灌注损伤的保护作用

目的观察苯那普利后处理对离体大鼠心肌缺血再灌注（I／R）损伤的保护作用，初步探讨其可能的作用机制。方法应用Langendroff离体灌流装置，采用完全停灌复灌方法制作离体大鼠心肌缺血再灌注模型。32只SD大鼠随机等分为4组：对照组、I／R组、缺血预处理组和苯那普利后处理组。测定各组稳灌20min和再灌60min时的冠脉流量，改良亮绿变色酸法（GCA）观察心肌损害程度，免疫组化法检测心肌组织中核因子-κB（NF—κB）和肿瘤坏死因子（TNF—α）的表达。结果与对照组比，I／R组，再灌注60min时的冠脉流量减少（P〈0．01），心肌损害程度增加（P〈0．01），免疫组织化学染色可见NF—κB蛋白表达显著增强且主要表达在心肌细胞核（P〈0．01），TNF—α主要表达在心肌细胞胞质，呈强阳性。与I／R组相比，苯那普利后处理组再灌注60min时的冠脉流量增多（4．3±0．4）ml／min和（3．5±0．5）ml／min，P〈0．05]，GCA染色心肌变性减轻，阳性率减低（14％±7％和40％±7％，P〈0．01），NF—κB表达减少（34．8％±4．7％和49．3％±9．7％，P〈0．05），TNF—α表达为弱阳性。苯那普利后处理组和预处理组相比较，差异无统计学意义（P〉0．05）。结论苯那普利后处理对缺血再灌注心肌具有保护作用，其机制可能与苯那普利后处理抑制NF—κB活性，减少TNF—α的表达有关。

Protective effects of benazepril postconditioning during ischenmia-reperfusion in isolated rats hearts

Objective To observe the protective effects of benazepril postconditioning during ischemia-reperfusion(I/R) in isolated rats hearts, and investigate the possible mechanism. Methods Thirty-two male Sprague-Dawley rats were isolated and perfused using the Langendorff mode with modified Kreb-Henseleit(KH) buffer and were randomly assigned into four groups: control group,I/R group, Preconditioning group, benazepril postconditioning group. The coronary flow was measured at minutes of perfusion 20 min and reperfu...