Ongoing pregnancies after intracytoplasmic injection using cryopreserved testicular spermatozoa

We report two clinical pregnancies occurring after intracytoplasmic sperminjection (ICSI) using cryopreserved spermatozoa obtained from testicularbiopsy, made in two different infertility situations in our clinic. Thefirst patient showed a secretory azoospermia associated with elevated serumfollicular stimulating hormone (FSH) level and spermiogenesis maturationarrest. The second patient was affected by azoospermia resulting frombilateral epididymal obstruction. Spermatozoa present in the wetpreparation of testicular biopsy made on the day of scrotal explorationwere cryopreserved within the testicular tissue for both men.Intracytoplasmic injections were performed at a later date, usingspermatozoa prepared from frozen-thawed tissues. In each case, threeembryos were obtained and transferred in utero. The transfers resulted in atwin pregnancy for the first case, and in a singleton pregnancy for thesecond. Living foetuses were seen in the ultrasound scan at the 7th weekand both pregnancies are proceeding to date beyond 30 weeks withoutcomplications.