Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells |
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| Authors: | Lance S. Terada Irene R. Willingham David M. Guidot Gayle N. Shibao Glenn W. Kindt John E. Repine |
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| Affiliation: | (1) Webb-Waring Lung Institute and Departments of Medicine and Neurosurgery, University of Colorado Health Sciences Center, 80262 Denver, Colorado |
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| Abstract: | Exposure to recombinant human tumor necrosis factor-alpha (TNF- ) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF- injures brain microvascular EC and that this effect may be mediated by xanthine oxidase. |
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