HPLC法同时测定不同产地白芷中2种香豆素的含量

目的采用HPLC法同时测定不同产地白芷中 2种香豆素的含量。方法色谱柱为岛津UL TRONVX ODS (2 5 0mm× 4 6mm ) ,5 μm柱 ,流动相为乙腈 水 (V∶V =6 5∶ 35 ) ,流速为0 8mL·min-1,检测波长 2 4 9nm。结果欧前胡素在 5～ 5 0mg·L-1(r =0 9999) ,异欧前胡素在2～ 2 0mg·L-1(r =0 9998)内线性关系良好 ,欧前胡素和异欧前胡素平均回收率分别为 98 4 %、96 3% ,RSD分别为 1 2 %、1 7%。结论不同产地白芷中 2种香豆素总量存在较大差异 ,可为评价不同产地白芷质量提供依据

Determination of the contents of two coumarins in Angelica dahurica from different regions by HPLC

Objective To determine the contents of two coumarins in Angelica dahurica from different regions by HPLC.Methods A ULTRON VX-ODS column(4.6 mm×250 mm,5 μm)was used with the mobile phase consisting of acetonitrile-water (V/∶V=65∶35,).The flow rate was 0.8 mL·min -1, and the detection wavelength was set at 249 nm.Results The linear ranges of imperatorin and isoimperatorin were 5～50 mg·L -1 (correlation coefficient r= 0.999 8) and 2～20 mg·L -1 (correlation coefficient r= 0.999 9),respectively.The average recoveries of imperatorin and isoimperatorin were 98.4%(RSD=1.2%) and 96.3% (RSD=1.7%), respectively.Conclusion There are great differences in the contents of two coumarines in Angelica dahurica from different regions.And the contents of two coumarines can provide a basis for the quality assessment for Angelica dahurica from different regions.