胆管癌p53基因突变和蛋白表达的研究

目的了解胆管癌ｐ５３基因突变和蛋白表达的变化。方法应用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）银染技术检测胆管癌中ｐ５３基因突变，并用亲和素－生物素－过氧化物酶复合物（ＡＢＣ）免疫组织化学染色方法检测胆管癌ｐ５３蛋白的表达。结果１１例胆管癌中，３例胆管癌样品ＰＣＲ－ＳＳＣＰ单链电泳带迁移异常，依据ＤＮＡ单链构象与分子电泳迁移的关系，胆管癌ｐ５３基因５～８外显子的突变率为２７％；６例有ｐ５３蛋白异常表达，阳性率为５５％。结论导致ｐ５３蛋白异常表达的ｐ５３基因突变是胆管癌的一种分子结构改变，可能在胆管癌的发生和发展中起着一定的作用。

MUTATION OF p53 GENE AND EXPRESSION OF p53 PROTEIN IN HUMAN CHOLANGIOCARCINOMA

To study the change of p53 gene mutation and protein expression in cholangiocarcinoma.METHODS We detected p53 gene mutation by PCR-SSCP staining technique and protein expression by ABC immunohistochemistry.RESULTS The abnormal shifting of PCR-SSCP single-strand DNA were observed in 3 out of 11 cases of cholangiocarcinoma. The data on the relationship between mobility and single strand conformation showed that the mutation rate in exon 5, 6, 7 and 8 of p53 gene from these cancer cases was 27%. Nuclear accumulation of p53 protein was observed in 6 out of 11 cases of cholangiocarcinoma(55% ).CONCLUSIONS p53 gene mutation, which results in abnormal protein accumulation,was a molecular structural change and may play a role in tumor generation and progression in human cholangiocarcer in cinoma.