| 高效液相色谱法同时测定氯地滴耳液中主药的含量 |
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| 引用本文： | 黄奠坤,雷鹏,戴智勇.高效液相色谱法同时测定氯地滴耳液中主药的含量[J].中国药房,2002,13(8):497-498. |
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| 作者姓名： | 黄奠坤 雷鹏 戴智勇 |
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| 作者单位： | 中南大学湘雅医院,长沙市410008 |
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| 摘 要： | 目的 :建立测定氯地滴耳液中氯霉素和地塞米松磷酸钠含量的高效液相色谱法。方法 :色谱柱为KromasilC8 柱 ,流动相为甲醇 -0 34 %磷酸二氢钾水溶液 (60∶40 ,V/V) ,检测波长为240nm。结果 :氯霉素在100～500μg/ml范围内浓度与峰面积线性关系良好 ,r=0 9999 ,回收率为98 5 %～100 8 % ,RSD≤1 5 % ;地塞米松磷酸钠在16～80μg/ml范围内浓度与峰面积线性关系良好 ,r=0 9990 ,回收率为98 8 %～100 6 % ,RSD≤2 0 %。结论 :该方法可用于同时测定氯地滴耳液中氯霉素和地塞米松磷酸钠的含量。
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| 关 键 词： | 高效液相色谱法 氯霉素 地塞米松磷酸钠 |
| 文章编号： | 1001-0408(2002)08-0497-02 |
| 修稿时间： | 2002年3月19日 |
Determination of Chloramphenicol and Dexamethasone Sodium Phosphate in Ear Drops by HPLC |
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| HUANG Diankun,LEI Peng,DAI Zhiyong.Determination of Chloramphenicol and Dexamethasone Sodium Phosphate in Ear Drops by HPLC[J].China Pharmacy,2002,13(8):497-498. |
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| Authors: | HUANG Diankun LEI Peng DAI Zhiyong |
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| Abstract: | OBJECTIVE:To establish a method for the simultaneous determination of chloramphenicol and dexamethasone sodium phosphate in ear drops by HPLC METHODS:Chloramphenicol and dexamethasone sodium phosphate were separated with a mobile phase of methanol-phosphate buffer solution(0 34% KH2PO4 solution)(60∶40,v/v,1 0ml/min)on Kromasil C8 column and detected at 240nm RESULTS:Good linearity appeared in the concentration of chloramphenicol 100～500μg/ml,r=0 9 999,the recovery was 98 5%～100 8%,RSD≤1 5%;and good linearity showed in the concentration of dexamethasone sodium phosphate 16～80μg/ml,r=0 9 990,the recovery was 98 8%～100 6%,RSD≤2 0% CONCLUSION:This method can simultaneously detect both ingredients in the ear drops |
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| Keywords: | HPLC chloramphenicol dexamethasone sodium phosphate |
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