血管内皮生长因子在原发性肝细胞癌中的表达及其作用

目的：探讨血管内皮生长因子（VEGF）的表达与原发性肝细胞癌（PHCC）侵袭和转移的关系。方法：采用免疫组织化学链酶卵白素－辣根过氧化物酶（LSAB）法对30例PHCC和20例癌旁组织手术切除后的石蜡标本组织中的VEGF表达及微血管密度（MVD）进行检测.结果 VEGF表达，MVD在PHCC组织中比癌旁组织明显增高，在PHCC中，有转移者及包膜不完整者VEGF表达，MVD明显高于无转移者及包膜完整者，VEGF表达，MVD在大肿瘤（直径＞5厘米）与小肿瘤（直径小于等于5厘米）两者间无显著性差异，VEGF的表达与MVD相关，结论：PHCC组织中的VEGF表达，MVD与肿瘤的侵袭和转移行为密切相关，可作为判定PHCC发生，转移及预后的指标，为监测和治疗提供了新思路。

The expression and clinical significance of vascular endothelial growth factor in primary hepatocellular carcinoma

Objective　To study the relationship between the expression of vascular endothelial growth factor (VEGF) and invasion, metastasis of primary hepatocellular carcinoma (PHCC). Methods　Paraffin-embedded specimens from 30 patients with PHCC undergoing radical resection were studied. Agiogenesis was assessed by the expression of VEGF and microvascular density (MVD) using LSAB immunohistochemical staining. Results　(1) The level of VEGF and MVD in　 PHCC was higher than that in the paratumorous tissue (P<0.05). (2) The level of VEGF expression and MVD in PHCC with tumor metastasis or in nonencapsulated tumors were higher than that in PHCC without metastasis or in well encapsulated tumors (P<0.05). 　(3) There was no significant differance in the expression of VEGF and MVD between large PHCC (diameter >5cm) and small ones (diameter ≤5cm). (4) The expression of VEGF was positively correlated with MVD (P<0.01).　　Conclusions　The expression of VEGF and MVD are highly correlated with invasion and metastasis in PHCC, may serve as a parameter for determining tumor metastasis and prognosis which may suggest a new idea to supervise and treat PHCC.