人博卡病毒广州分离株全基因组序列测定及分析

目的 探讨广州地区儿童感染的博卡病毒基因组分子结构特点和基因型类型.方法 参考GenBank上的博卡病毒BJ3064株(DQ988933)基因组设计分段扩增引物,进行PCR分段扩增博卡病毒基因组,PCR产物直接送测序公司测序,序列测定,用ClustalW/X和MEGA5.0等软件分析基因组序列.结果 博卡病毒全基因组为...

Detection and analysis on complete genomic sequence of human bocavirus GZ2010-1 strain in Guangzhou

Objective To study the structural features and different types of the genomic molecular organization of human bocavirus in infected infants in Guangzhou. Methods Primers were designed on the basis of the genomic sequence of human bocavirus BJ3064 strain (DQ988933) in the GenBank, and amplify HBOV genome by PCR.The PCR-products were directly sequenced. The genomic nucleotide sequences were analyzed with the programs of CLUSTAL W/X and MEGA5.0.Results The strain GZ2010-1 genome is 5299bp in length. The genomic sequences of GZ2010-1 strain were compared with those sequences of HBOV1, HBOV2, HBOV3, and HBOV4 genotypes in GenBank, revealed that the homology with HBOV1 genotype ranged from 98% to 99%, homology with genotypes HBOV2, HBOV3, HBOV4 were 76%, 81% ,and 75% respectively. The F genes of GZ2010-1 strain was compared with F genes of HBOV1 genotype, showed that the homology was also between 98%-99%. Conclusions The complete nucleotide sequence of human bocavirus GZ2010-1 strain isolated from Guangzhou is 5299 bp in length,GenBank accession NO.is JN 128956. Sequence and phylogenetic analysis of the GZ2010-1 strain reveales that this isolate belongs to HBOV1.