PDCD5蛋白在胃腺癌及其癌前病变中的差异性表达研究

目的：通过检测程序化细胞死亡因子5（PDCD5）在慢性浅表性胃炎、慢性萎缩性胃炎伴肠上皮化生、慢性萎缩性胃炎伴不典型增生及胃腺癌中的表达，以探讨PDCD5蛋白在胃腺癌发生发展过程中的重要作用。方法应用Envision免疫组织化学方法，检测PDCD5在30例慢性浅表性胃炎、30例萎缩性胃炎伴肠上皮化生、30例萎缩性胃炎伴不典型增生和30例胃腺癌组织中的表达。统计学处理应用SPSS19.0统计软件进行。结果①PDCD5在慢性浅表性胃炎中表达最高，明显高于癌前病变及癌组织中的表达（P＜0.05）；在肠化组织中的表达明显高于不典型增生组和胃腺癌组（P＜0.05），在中重度不典型增生和胃腺癌中的阳性率差异无显著性（P＞0.05）。②PDCD5在胃腺癌组织中的表达与胃癌的组织病理分级无显著相关性（P＞0.05）。结论 PDCD5在慢性浅表性胃炎‐慢性萎缩性胃炎伴肠上皮化生‐慢性萎缩性胃炎伴不典型增生‐胃腺癌的过程中阳性表达逐渐降低；但其在胃腺癌中的阳性表达与肿瘤的组织分化程度无关。

The differential expression of PDCD5 protein in human gastric cancer and precancerous lesions

Objective To investigate the effect of PDCD5 in gastric cancer through detecting and analysing PDCD5 expression in chronic superficial gastritis ,atrophy with intestinal metaplasia ,atrophy with atypical hyperplasia and gastric adenocarcinoma . Methods The expression of PDCD5 protein in 30 cases of chronic superficial gastritis ,30 cases of atrophy with intestinal meta‐plasia ,30 cases of atrophy with atypical hyperplasia and 30 cases of gastric carcinoma were detected by Envision immunohisto‐chemical technique .These values were statistically compared with SPSS19 .0 .Results ①The tissues of chronic superficial gas‐tritis showed highest PDCD5 expression ,which was significantly higher than that of precancerous lesions or cancer tissues(P<0.05);The positive rate of PDCD5 in the intestinal metaplasia group was much higher than that of dysplasia and gastric carcino‐ma (P<0.05) .There was no significant difference between moderate severe dysplasia and carcinoma (P>0.05) .②There was no significant difference of PDCD5 expression in the differentiation degree of gastric carcinoma(P> 0.05) .Conclusion The positive rate of the expression of PDCD5 in the process of chronic superficial gastritis‐chronic atrophy with intestinal metapla‐sia‐chronic atrophy with atypical hyperplasia‐gastric carcinoma is gradually decreased ,although the expression of PDCD5 in gas‐tric carcinoma is not related to the differentiation degree of the tissue .