| pEGFP-HPV16E6/E7表达载体的构建及转染角朊细胞的瞬时表达 |
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| 引用本文： | 廖文俊,刘彦仿,刘玉峰.pEGFP-HPV16E6/E7表达载体的构建及转染角朊细胞的瞬时表达[J].第四军医大学学报,1999,20(8):659-660. |
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| 作者姓名： | 廖文俊 刘彦仿 刘玉峰 |
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| 作者单位： | 1. 第四军医大学西京医院皮肤科,陕西,西安,710033
2. 第四军医大学基础部病理学教研室,陕西,西安,710033 |
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| 基金项目： | 国家自然科学基金!39570656 |
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| 摘 要： | 目的：构建ｐＥＧＦＰ－ＨＰＶ１６Ｅ６／Ｅ７表达载体,观察其在角朊细胞中的瞬时。方法：基因重组技术,基因转染技术。结论 ｐＥＧＦＰ－ＨＰＶ１６Ｅ６／Ｅ７表达载体便于观察,转染细胞中ＨＰＶ１６Ｅ６／Ｅ７－ＧＦＰ融合蛋白的表达民政部及蛋白定位,适用于对ＨＰＶ１６Ｅ６／Ｅ７分子生物学特性及致瘤机理的研究。
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| 关 键 词： | 人乳头瘤病毒 表达载体 构建 基因转染 |
| 修稿时间： | 1998-09-02 |
Construction of pEGFP-HPV16E6/E7 expression vector and detection of transient expression in transfected keratinocytes |
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| LIAO Wen-Jun,LIU Yan-Fang,LIU Yu-Feng.Construction of pEGFP-HPV16E6/E7 expression vector and detection of transient expression in transfected keratinocytes[J].Journal of the Fourth Military Medical University,1999,20(8):659-660. |
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| Authors: | LIAO Wen-Jun LIU Yan-Fang LIU Yu-Feng |
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| Abstract: | AIM: To construct PEGFP--HPV16E6/E7 expression vector and to detect its transient expression in transfected keratinocytes. METHODS: Techniques of gene recombination and gene transfection were employed. RESULTS: identification of PEGFP HPV16E6/E7 by enzyme digestion showed that HPV16E6/E7 fragment had been cloned into EcoR 1 and BamH I sites of PEGFP N3 and the expression of GFP in transfected keratinocytes was observed. CONCLUSION: pEGFP--HPV16E6/E7 expression vector makes it easyto assess the expression of HPV16E6/E7--GFP fusion proteinand the protein localization in trans feeted cells. |
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| Keywords: | human papillomavirus expression vector construction gene transfection |
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