高效液相色谱法测定苯巴比妥、苯妥英、卡马西平的血药浓度

目的 :建立 HPL C法测定抗癫药苯巴比妥 (PB)、苯妥英 (PT)、卡马西平 (CBZ)的血药浓度。方法 :反相柱 ODS- Hy-persil(4.6 m m× 10 0 mm ,5︼m ) ,流动相为甲醇∶水 (5 2∶ 48) ,流速 0 .8m l/ m in,紫外检测波长 2 5 4nm ,以上 3药互为内标。标本经 CH2 Cl2 提取 ,蒸干后用流动相重溶进样。结果 :PB、PT、CBZ的保留时间分别为 3.2 2、5 .5 7、6 .80 m in;最低检测浓度分别为 0 .2 5、0 .5、0 .0 5︼g/ m l;线性范围分别为 2 .5～ 40、2 .5～ 40、1.2 5～ 2 0︼g/ ml;相对回收率分别为 10 1.49%、10 4.19%、98.70 % ;日内 RSD分别为 1.81%、5 .94%、1.81% ;日间 RSD分别为 6 .0 6 %、3.35 %、3.96 %。结论 :本法具快速、灵敏、实用等优点。

Determination of phenobarbital, phenytoin, carbamazepine in serum by HPLC

AIM: To develop a method for determination of phenobarbital (PB),phenytoin (PT), carbamazepine (CBZ) in serum by high performance liquid chromatography (HPLC) for providing a scientific basis for clinical use of antiepileptic drugs. METHODS: 0.25 ml serum sample was extracted by 2 ml CH2Cl2, dissolved by mobile phase. ODS Hypersil (4.6 mm×100 mm, 5 μm) column was used；mobile phase: MeOH-H2O (52∶48), flow rate 0.8 ml/min. Ultraviolet detection wavelength was 254 nm. PB, PT and CBZ were complete internal standard each other. RESULTS: The parameters of PB PT and CBZ were as follows: tR 3.22, 5.57, 6.80 min；the lowest detection concentration 0.25, 0.5, 0.05 μg/ml respectively. The linear relation was in the range of 2.5-4.0， 2.5-40 and 1.25-20 μg/ml. Relative extraction recovery rate were 101.49%, 104.19% and 98.70%. The intra-day precision were 1.81%, 5.94%, 1.81% respectively. The inter-day precision were 6.06%, 3.35%, 3.96% respectively. CONCLUSION: The method is rapid, sensitive and practical.