复方小柴胡汤对荷EAC鼠IL-2水平和CD4+/CD8+比值的影响

目的： 探讨复方小柴胡汤（FFXCHT）对艾氏腹水癌（EAC）荷瘤小鼠脾细胞IL-2水平和血CD4+/CD8+比值的影响。方法： 观察FFXCHT对EAC荷瘤小鼠的肿瘤重量、胸腺和脾系数的影响；用［3H］-TdR掺入法测定脾细胞IL-2的水平；用流式细胞仪测定血CD4+和CD8+T淋巴细胞含量，并计算CD4+/CD8+比值。结果： FFXCHT高、中和低浓度治疗组EAC荷瘤小鼠肿瘤重量均明显少于模型组（P<0.01）。模型组胸腺系数和脾系数高于空白对照组（P<0.05）。FFXCHT高、中和低浓度治疗组脾系数和胸腺系数均明显高于模型组（P<0.01）。与模型组相比，FFXCHT高、中和低浓度治疗均能明显提高荷瘤鼠脾细胞IL-2分泌水平（P<0.05）和血CD4+/CD8+ T淋巴细胞比值（P<0.05）,其中以中浓度治疗组最为明显。结论： FFXCHT能明显抑制EAC肿瘤的生长，提高胸腺和脾系数、脾细胞IL-2活性及血CD4+/CD8+比值，提示FFXCHT可能通过调整EAC荷瘤小鼠免疫功能而发挥其抗肿瘤作用。

Influence of Fu Fang Xiao Chai Hu Tang on level of interleukin-2 and value of CD4+/CD8+ in mice bearing Ehrlish ascites carcinoma

AIM:To investigate the effects of Fu Fang Xiao Chai Hu Tang(FFXCHT)on level of Interleukin-2 and value of CD4 /CD8 in mice bearing Ehrlish ascites carcinoma(EAC).METHODS:The effects of FFXCHT on the EAC were observed and index of thymus and spleen were observed.The method of [3H]-TdR incorporation was used to measure the IL-2 level,and the value of CD4 /CD8 was assayed by ELITE calibur flow cytometry.RESULTS:Compared with the model group,FFXCHT inhibited the growth of EAC(P<0.01).The index of thymus and spleen of the model group were higher than that in the control group(P<0.05).Compared with the model group,FFXCHT increased the index of thymus and spleen,the IL-2 level and the value of CD4 /CD8 (P<0.05),especially with the middle dose.CONCLUSION:The FFXCHT has antitumor effect.Increased IL-2 level and the value of CD4 /CD8 play a regulatory role in the immunologic function in mice bearing EAC.