The effect of 2-amino-4-phosphonobutyrate (APB) on acetylcholine release from the rabbit retina: evidence for on-channel input to cholinergic amacrine cells |
| |
| Authors: | M J Neal J R Cunningham T A James M Joseph J F Collins |
| |
| Institution: | 1. Department of Pharmacology, School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX U.K.;1. Department of Chemistry, Sir John Cass School of Science and Technology, City of London Polytechnic, London EC2N 2EY U.K. |
| |
| Abstract: | The light-evoked release of acetylcholine (ACh) from the rabbit retina was taken as a measure of cholinergic amacrine cell activity. The glutamate analogue DL-(+/-)-2-amino-4-phosphonobutyric acid (APB) prevented the light-evoked release of ACh and also selectively abolished the ON-responses of ganglion cells and the ERG b-wave. It is concluded that the input to cholinergic amacrine cells involves mainly the depolarizing bipolar cells, which subserve ON-channels. L-(+)-stereoisomer of APB was 15 times more potent than the D-(-)-isomer in suppressing ACh release and the b-wave, suggesting that the mechanism of action of APB does not involve antagonism of excitatory amino acids. |
| |
| Keywords: | To whom correspondence should be addressed at: Department of Pharmacology University of Sydney NSW 2006 Australia |
| 本文献已被 ScienceDirect 等数据库收录！ |
