纳米雄黄对卵巢癌细胞增殖凋亡的调控作用及分子机制研究

目的：探讨纳米雄黄对卵巢癌细胞Skov3增殖和凋亡的影响及其潜在分子机制。方法：采用机械研磨法制备纳米雄黄；以Skov3细胞为靶细胞，分别采用MTT实验及流式细胞术检测细胞增殖能力和凋亡情况；同时，利用Western blot检测细胞内Bcl-2及Bax蛋白的表达变化。结果：纳米雄黄能够显著抑制细胞增殖能力；40mg/L和80mg/L纳米雄黄处理Skov3细胞48小时，细胞凋亡显著增加；40mg/L纳米雄黄处理Skov3细胞48小时，Skov3细胞内Bcl-2蛋白表达水平显著降低，Bax蛋白表达显著增加。结论：纳米雄黄能够显著抑制Skov3细胞增殖，促进其凋亡，可能通过抑制细胞内Bcl-2蛋白的表达，上调Bax蛋白表达发挥抑制卵巢癌的发生发展的作用。

The regulation of Realgar Nanoparticle on the proliferation and apoptosis of human ovarian carcinoma cell and the underlying mechanisms

Objective To elucidate the effects of Realgar Nanoparticle on the proliferation and apoptosis of SKOV3 human ovarian carcinoma cells and the underlying mechanisms. Methods Preparation of Realgar Nanoparticle was mechanical milled using a high-energy planetary ball mill. The MTT assay and flow cytometry were used to evaluate the proliferation and apoptosis of SKOV3 cells, respectively. Western blot was employed to determine the expression level of Bcl-2 and Bax in SKOV-3 cells. Results Realgar Nanoparticle could significantly inhibit the proliferation of SKOV3 cells, and treating SKOV3 cells with Real-gar Nanoparticle (40mg/L and 80mg/L) resulted in significantly increased apoptosis. After being incubated with the Realgar Na-noparticle (40mg/L) for 48h, the expression level of Bcl-2 was significantly reduced and Bax expression level was significantly increased in the SKOV3 cells. Conclusion Realgar Nanoparticle can inhibit the proliferation and induce the apoptosis of Skov3 cells, and the inhibitory effects of Realgar Nanoparticle on ovarian carcinoma are probably exerted by decreasing the expression of Bcl-2 protein and increasing the expression of Bax protein.