薯蓣皂苷元对肺动脉高压大鼠肺血管重构的影响

目的：探讨薯蓣皂苷元（Dio）对野百合碱（MCT）诱导的肺动脉高压大鼠肺血管重构的影响。方法：将30只雄性 SD 大鼠随机分为正常对照组、MCT 组和 Dio 组，每组10只。MCT 组和 Dio 组一次性腹腔注射野百合碱60 mg/kg，建立大鼠肺动脉高压模型。Dio 组每天给予 Dio 80mg/kg 灌胃，对照组和 MCT 组给予相同量溶剂灌胃。待药物干预满4周后，分别测量各组干预后平均肺动脉压力（mPAP）、右心室肥大指数（RVHI）、肺小动脉管壁厚度占管径的百分比（WT％）和管壁面积占血管总面积的百分比（WA％）；采用免疫组化检测肺组织核因子-κB（NF-κB）的表达。结果：MCT 组的 mPAP、RVHI、WT％、WA％及 NF-κB 的表达水平均显著高于对照组和 Dio 组；经薯蓣皂苷元干预后，mPAP、RVHI、WT％、WA％及 NF-κB 的表达水平均明显降低。结论：薯蓣皂苷元能够缓解 MCT诱导的肺动脉高压大鼠肺血管重构，其机制可能与抑制 NF-κB 的表达有关。

Effect of diosgenin on pulmonary vascular remodeling in pulmonary hypertension in rats

Objective To evaluate the effects of diosgenin on monocrotaline-induced pulmonary arterial remodeling in rats. Methods 30 male SD rats were randomLy divided into the normal control group,the MCT group,the Dio group,with ten rats in each group.The rats in MCT group and Dio group were given a dose of 60 mg/kg monocrotaline intraperitoneally in PAH.Di-osgenin (80mg/kg) were given orally for to the Dio group,and the same amount of solvent were given orally to the control group and the MCT group.4 weeks after medication,mean of pulmonary arterial pressure(MPAP)and right ventricular hypertrophy index(RVHI)were measured,and WT% and WA% of pulmonary arterioles were evaluated.The expression of factor -kappaB (NF-κB) in lung tissue was measured by immunohistochemistry. Results mPAP,RVHI,WT%,WA%,and the expression of NF-κB were increased significantly in MCT group than those in control group and Dio group. After intervention of diosgenin , mPAP,RVHI,WT%,WA%,and the expression of NF-κB were significantly reduced. Conclusion Diosgenin could alleviate pulmonary hypertension pulmonary vascular remodeling induced by monocrotaline in PH rats,and mitigate pulmonary vascular remodeling,and its mechanism may be related to inhibiting the expression of NF-κB .