多器官细胞共同培养方法检测双黄连口服液的细胞毒性

目的：观察双黄连口服液对人类体外不同靶器官细胞的毒性及特点。方法：应用人类多器官细胞共培养模型，观察双黄连口服液分别作用24、48、72 h后，对WI-38、HepG2、HEK293、SH-SY5Y和HCF细胞增殖的影响。结果：双黄连口服液处理24、48、72 h后，各种细胞的增殖率有随着剂量的降低而升高的趋势、且有较好的剂量-效应关系。该药除在72 h后的1/32浓度对HEK293细胞无影响外，在所有时点的各浓度均抑制该细胞的增殖。处理24 h后，1/4～1（原液）浓度的双黄连口服液抑制WI-38细胞，1/8～1（原液）浓度的该药抑制HepG2、SH-SY5Y和HCF细胞增殖；48 h后，1/16～1（原液）浓度抑制WI-38、HepG2、SH-SY5Y和HCF细胞；72 h后，1/2～1（原液）浓度的双黄连口服液抑制WI-38细胞，所有浓度的该药均抑制HepG2、SH-SY5Y和HCF细胞。结论：双黄连口服液对WI-38、HepG2、HEK293、SH-SY5Y和HCF细胞可能均有毒作用，其可能的毒性大小顺序为肾细胞>肝细胞>神经、心肌细胞>肺细胞。

Investigating cytotoxicity induced by Shuanghuanglian oral liquid with the human IdMOC model

Objective To observe the cytotoxicity and its features, induced by Shuanghuanglian oral liquid, of human's differ-ent target organs cells cultured in vitro. Methods After the human IdMOC(integrated discrete multiple organ cell culture)model was administrated with Shuanghuanglian oral liquid for 24 hours, 48 hours and 72 hours, the growth of WI-38, HepG2, HEK293, SH-SY5Y and HCF cells was detected. Results After Shuanghuanglian oral liquid was treated the cells for 24, 48, 72 hours re-spectively, the relative growth rates of all kinds of cells were increased with the drug’s concentration reducing, and there is a good dose-response relationship. Except that there is no effect on HEK293 cells induced by only the concentration of 1/32 of the drug after treated 72 hours, all kinds of cells proliferation was inhibited by all different concentration of Shuanghuanglian oral liquid at all time-points. After administrated by Shuanghuanglian oral liquid for 24 hours respectively, the proliferation of WI-38 cells was inhibited among the concentration of 1/4~1 of this drug, and that of HepG2, SH-SY5Y and HCF cells was among 1/8~1. The proliferation of WI-38, HepG2, SH-SY5Y and HCF cells was inhibited among the concentration of 1/16~1 of Shuanghuanglian oral liquid after treated 48 hours, and after 72 hours the proliferation of WI-38 cells was inhibited between the concentration of1/2~1, and that of HepG2, SH-SY5Y and HCF cells was inhibited by all different concentration of this drug. Conclusion There might be cytotoxicity of Shuanghuanglian oral liquid to WI-38, HepG2, HEK293, SH-SY5Y and HCF cells, and the possible tox-icity order of this drug was renal cells>hepatocytes>neural cells, cardiomyocytes>lung cells.