脂质体介导的p53基因对肝癌细胞生长的抑制作用

目的 观察外源野生型ｐ５３基因在肝癌基因治疗方面的可行性。方法 将载有人野生型ｐ５３－ｃＤＮＡ的真核表达质粒ｐ５３－ｐｃＤＮＡ３，用阳离子脂质体介导转染人肝癌细胞系ＨｅｐＧ２，用流式细胞仪检测ｐ５３－ｐｃＤＮＡ３对ＨｅｐＧ２细胞生长的影响。结果 通过观察细胞生长曲线与流式细胞仪检测细胞周期和细胞的凋亡指数发现，ＨｅｐＧ２细胞生长受到明显的抑制。结论 脂质体介导的ｐ５３基因可在Ｈ细胞中表达，且明显抑

Inhibitory effects of lipofectamin-mediated p53 gene on the growth of liver cancer cells

ObjectiveTo study the feasibility of the use of exogenous wild type p53 in gene therapy for liver cancer.MethodsRecombinant eukaryotic expressing plasmid p53 pcDNA 3 containing human wild type p53 cDNA was transiently introduced into human liver cancer cell line HepG2 mediated by cation iron lipofectamin and selected by G418.By flowcytometric analysis,the effects of p53 pcDNA 3 on the growth of liver cancer cells were evaluated.ResultsThe cell growth curve,cell cycle, apoptosis index of HepG2(control cells) were compared with those of HepG2 p53 cells.The growth of HepG2 p53 cells was greatly suppressed.ConclusionExogenous wild type p53 gene may stably exist in HepG2 cell after being mediated with lipofectamin.These results indicate that recombinant plasmid expressing wild type p53 may be useful for gene therapy of human liver cancer.