针刺抑制幼鼠HIBD脑组织神经细胞凋亡的实验研究

目的探讨针刺对新生大鼠缺血缺氧性脑损伤(HIBD)脑神经元调亡的保护作用及其机制。方法 (1)选用85只7 d龄清洁级SD大鼠,将大鼠结扎左侧颈总动脉后恢复2 h,置于透明密闭容器中,并入37℃恒温水中以1 L/min的速度通入低氧气体,2.5 h后将动物取出,存活者继续作行为测定,获成功模型72只,随机分为3组,A组为HIBD+针刺Ⅰ组,B组为HIBD+针刺Ⅱ组,C组为HIBD对照组;另设假手术组D组,每组24只。(2)选用幼鼠百会、患侧颞Ⅰ针、内关、曲池、足三里、涌泉,A组于造模后24 h开始针刺,前7 d仅针四肢部穴位,第8天开始加针头部穴位;B组造模后第8 d开始针刺,头、体针同步。针刺均为1次/d,A组连续针刺20 d,B组连续针刺13 d,假手术组干预措施与A组相同,至HIBD后第21天处死取材。采用TUNEL法测定针刺对HIBD大鼠额叶与海马区组织神经元凋亡,用图像分析仪在光镜下计算神经细胞凋亡数目。结果 4组左侧海马CA1区神经元及左侧大脑额叶皮质神经元凋亡数差异有统计学意义(P<0.05),其中D

Study on the effect of acupuncture inhibit the neuronal cell apoptosis in brain tissue of HIBD newborn rats

Objective To study the effect of acupuncture inhibit the neuronal cell apoptosis in brain tissue of hypoxiaischemic brain damage(HIBD) newborn rats.Methods Eighty-five clean conventional SD rats,aged 7 days,were selected.The rats was operated by ligation of left common carotid artery and recover for 2 hours.Then they were put in hyaline airtight vessel in the homeothermal water of 37 ℃.Hypoxia gas was infused to the vessel at the speed of 1 L/min.The animal were took out after 2.5 hours.The successful models were evaluated by the act of inability of roll over,abnormal balance or turning left.Seventy-two successful HIBD models were divided randomly to 3 groups.Group A was HIBD+acupuncture Ⅰ.Group B was HIBD+acupuncture Ⅱ.Group C was the control group of HIBD.Sham-operated group was set up to group D in addition.The number of the rats in each group was 24.The acupoints of Baihui,Ⅰneedle of temple in the affected lateral,Neiguan,Quchi,Zusanli,Yongquan were selected.Group A was acupunctured at 24 hours after the models were made.Only four extremities were acupunctured in initial 7 days.The head-acupoints were acupunctured in addition at 8th day.Group B began to be acupunctured at 8th day after the models were made.Head and body acupuncture were operated at the same time.In both of the two groups,the acupuncture operated once a day.Group A continued 20 days.Group B continued 13 days.Sham-operated group was interfered as same as group A.The rats were put to death 21 days after the HIBD.Neuronal cell apoptosis in the temple and hippocampus of HIBD rat were observed by TUNEL assay.The cell number of neuronal apoptosis was counted by the equipment of picture analysis at the light microscope.The difference was analyzed by analysis of variance between the groups.Results The difference of cell number of neuronal apoptosis in the CA1 zone of the left hippocampus and in the left temple of all the four groups has significance in statistics,D