Measurement of beta-2-microglobulin in serum and plasma by an enzyme-linked immunosorbent assay (ELISA) |
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| Authors: | O W Bjerrum H S Birgens |
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| Affiliation: | 1. Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Sakai, Osaka, Japan;3. Environmental Health Division, Health and Medical Care Office, Department of Health and Public Welfare, Nagoya, Aichi, Japan;4. Institute of Social Welfare and Public Health, Nishi-, Kasugai, Aichi, Japan;5. Department of Infectious Diseases, Kobe Institute of Health, Kobe, Japan;6. Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan;7. The Global Station for Zoonosis Control, Hokkaido University Global Institution for Collaborative Research and Education, Sapporo, Japan;8. United Graduate School of Veterinary Sciences, Gifu University, Yanagido, Gifu, Japan;9. Department of International Health, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan;10. School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki, Japan;1. Université Paris-Est, Laboratoire National de Référence Tuberculose, Unité Zoonoses Bactériennes, Laboratoire de Santé Animale, ANSES, 94706 Maisons-Alfort Cedex, France;2. INRA, Université de Tours, UMR1282, Infectiologie et Santé Publique, F-37380 Nouzilly, France |
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| Abstract: | A simple technique for the measurement of beta-2-microglobulin (beta 2M) in serum was developed. The method was designed as a sandwich technique using rabbit anti-human antibodies, employing commercially available reagents in an enzyme linked immunosorbent assay (ELISA). The assay was of high specificity, sensitivity, accuracy and reproducibility. beta 2M in serum was strongly correlated with age (p less than 0.005), but independent of sex. Values in heparin and citrate plasma were significantly lower than in serum (p less than 0.001), whereas values in serum and EDTA plasma were similar. Release of beta 2M from normal blood cells was not observed in vitro before the test procedure. An excellent correlation between the results obtained in the ELISA and a RIA was demonstrated (rS = 0.99, p less than 0.0001). |
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