丹参抑制泼尼松大鼠骨质丢失

目的:探讨长期超生理剂量应用泼尼松对大鼠所致的骨质疏松症特点,同时观察中药丹参对其防治作用。方法:24只♂SD大鼠随机分成正常对照组,泼尼松组和丹参组,每组8只。正常对照组给予生理盐水处理,其他组先予泼尼松按2.7 mg/kg ig,随后泼尼松组灌喂生理盐水,丹参治疗组按5.0 g/kg灌喂丹参水提液。每日1次,连续12周。实验结束后用骨组织形态计量学等方法测量股骨的动态参数和静态参数,同时测量尺骨的羟脯胺酸和钙磷含量以及测量股骨的长度和宽度。结果:泼尼松大鼠骨量显著丢失及骨生长受到抑制(P<0.01),而丹参治疗组可明显对抗泼尼松对骨的生长抑制作用。结论:4月龄SD大鼠服用泼尼松12周后可造成骨质疏松症,丹参对其有较好的对抗作用。

Effect of Salvia miltiorrhiza Bge on glucocorticoid-induced osteoporois in rats

AIM:To study the (rat's) osteoporosis induced by long-term administration of prednisone,and investigate the prophylactic effects of Salvia miltiorrhiza Beg in male rats. METHODS: Twenty-four SD rats were randomly divided into four groups such as control group,prednisone group and Danshen(Salvia miltiorrhiza Bge) group,with 8 rats per group.control group was treated with normal saline,other groups with prednisone at dose of(2.7) mg/kg first,and then prednisone group with normal saline group with Salvia miltiorrhiza Bge at dose of 5.0 g/kg,respectively,once a day for 12 weeks.At the experimental endpoint,bone histomorphometric analysis of thighbone were performed in undecalcified sections,the contet of Ca~(2 ) and oxyproline hydroxyproline of ulnar bone were to be measured.length and width of the thighbone were to be tested. RESULTS: Bone histomorphometric parameters showed bone loss was significant to prednisone group(P<0.01).Compared with prednisone group,Danshen group could prevent bone loss markedly(P<0.05). CONCLUSION: Danshen root has good protective effect on bone loss occured in four month-old male Sprague-Dawley rats after prednisone-treated for 12 weeks.