Establishment,growth properties,and morphological characteristics of permanent human small cell lung cancer cell lines

Summary Cell lines from SCLC were established with a success rate of 43% from different metastatic sites of treated and untreated patients. All 6 SCLC cell lines grew as floating cell aggregates without substrate adherence. The degree of aggregation ranged from very tight spheroids to very loose sheets and chains. This gross morphological property showed a striking correlation to the PDT, with short PDTs in loose growing cell lines and long PDTs in tight growing cell lines. Cell size and nuclear features, i.e., chromatin pattern and nucleolar prominence, also seemed to correlate with the PDT and gross morphology. All SCLC cell lines had dense core granules by electron microscopical examination. Several different serum-free and serum-supplemented growth media were tested for their feasibility in estabilishing and permanently growing SCLC. Serum-free SIT medium and SIT 2.5 medium provided the best results in liquid culture. For semisolid SCLC cultivation, R10 medium was suprior to all other media tested. These cell lines are currently under intensive biochemical, molecular biological, and cytogenetical investigation in different laboratories and thus provide a tool for studying the biology of lung cancer.Abbreviations SCLC small cell lung cancer - FBS fetal bovine serum - R 10 Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% FBS - D 10 Dulbecco's modified Eagle's medium supplemented with 10% FBS - SIT RPMI 1640 medium supplemented with selenium, insulin, transferrin - HITES RPMI 1640 medium supplemented with hydrocortisone, insulin, transferrin, estradiol, selenium - SIT 2.5 SIT medium supplemented with 2.5% FBS - PBS phosphate buffered saline - PDT population doubling timeThis work was supported by the SFB 215 of the German Research Society. The authors Thank Dr. Adi F. Gazdar for reviewing the morphology