| RP-HPLC法测定固体脂质纳米粒中β-榄香烯的含量 |
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| 引用本文： | 王艳芝,金圣煊,邓意辉,郑甲信,毕殿洲.RP-HPLC法测定固体脂质纳米粒中β-榄香烯的含量[J].沈阳药科大学学报,2005,22(4):286-288. |
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| 作者姓名： | 王艳芝 金圣煊 邓意辉 郑甲信 毕殿洲 |
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| 作者单位： | 1. 沈阳药科大学,药学院,辽宁,沈阳,110016
2. 沈阳绿洲药业有限公司,辽宁,沈阳,110015 |
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| 摘 要： | 目的建立固体脂质纳米粒注射液中β榄香烯的反相高效液相色谱法。方法色谱柱:HypersilBDSC18(4.6mm×200mm,5μm);流动相:乙腈水(体积比为88∶12);检测波长:210nm;柱温:30℃。结果β榄香烯与其他组分分离良好,线性范围10.6～53.0mg·L-1(r=0.9999),平均回收率为99.6%,RSD为0.93%(n=5)。结论方法简便、准确,可作为该制剂的质量控制方法。
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| 关 键 词： | β-榄香烯 反相高效液相色谱法 固体脂质纳米粒 |
| 文章编号： | 1006-2858(2005)04-0286-03 |
| 修稿时间： | 2004年11月12 |
Determination of β-elemene in solid lipid nanoparticles by RP-HPLC |
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| WANG Yan-zhi,JIN Sheng-xuan,DENG Yi-hui,ZHENG Jia-xin,BI Dian-zhou.Determination of β-elemene in solid lipid nanoparticles by RP-HPLC[J].Journal of Shenyang Pharmaceutical University,2005,22(4):286-288. |
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| Authors: | WANG Yan-zhi JIN Sheng-xuan DENG Yi-hui ZHENG Jia-xin BI Dian-zhou |
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| Institution: | WANG Yan-zhi~1,JIN Sheng-xuan~1,DENG Yi-hui~1,ZHENG Jia-xin~2,BI Dian-zhou~1 |
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| Abstract: | Objective To develop a RP-HPLC method for the determination of β-elemene in solid lipid nanoparticles(SLN) injection.Methods The separation was carried out on Hypersil BDS C(18) ((5 μm),(4.6 mm×) (200 mm)) by using acetonitrile-water (88∶12) as the mobile phase with detection at (210 nm) and the column temperature was set at (30 ℃).Results β-Elemene can be well separated from other components,with the linear range of 10.6～ (53.0 mg·L~(-1)) ((r=) (0.999 9)),the average recovery of 99.6% (RSD of 0.93%,(n=3).)Conclusion This method is accurate and reliable for the determination of β-elemene in SLN. |
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| Keywords: | β-elemene RP-HPLC solid lipid nanoparticles |
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