| 梯度洗脱法测定心可舒颗粒中葛根素的含量 |
| |
| 引用本文： | 王文渊,张芸兰.梯度洗脱法测定心可舒颗粒中葛根素的含量[J].湖北民族学院学报(医学版 ),2009,26(4):20-22. |
| |
| 作者姓名： | 王文渊 张芸兰 |
| |
| 作者单位： | 永州职业技术学院,湖南,永州,425006 |
| |
| 摘 要： | 目的探讨心可舒颗粒中葛根素含量测定的方法。方法采用反相高效液相色谱法,Alltima-C18色谱柱（5μm,4.6×150 mm）;以甲醇-0.2%磷酸二氢钾溶液为流动相进行梯度洗脱,流速为1.0 mL/min;检测波长250nm;柱温25℃。结果葛根素在6.38-63.8μg/mL的范围内与峰面积呈良好的线性关系（r=0.9997）,平均回收率为97.33%,RSD为1.59%（n=5）。结论该方法简便、准确,重现性好,可更好的控制心可舒颗粒的质量。
|
| 关 键 词： | 葛根素 心可舒颗粒 含量测定 |
Determination the Content of Puerarin in Xinkeshu Granule by Gradient Elution Method |
| |
| WANG Wen-yuan,ZHANG Yun-lan.Determination the Content of Puerarin in Xinkeshu Granule by Gradient Elution Method[J].Journal of Hubei Institute For Nationalities(Medical Edition),2009,26(4):20-22. |
| |
| Authors: | WANG Wen-yuan ZHANG Yun-lan |
| |
| Institution: | WANG Wen-yuan,ZHANG Yun-lan(Yongzhou Vocational Technical College,Yongzhou 425006,China) |
| |
| Abstract: | Objective To research a method for determination of Puerarin in Xinkeshu Granules.Methods Gradient elution method was used to determine the content of Puerarin.The analytical column was Alltima-C18（5μm,4.6×150mm）,with Methanol-0.2% potassium primary phosphate Solution as amobile phase,the column temperature was 25℃,the detection wavelength was 250nm and the flow rate was 1.0ml/min.Results The calibration curve was linear over the rage of 6.38～63.8μg/mL（r=0.9997）,the average recovery was 97.33% with 1.59% RSD（n=5）.Conclusion The method was simple,accurate and reliable.It can be used for the quality control for Xinkeshu Granules. |
| |
| Keywords: | Puerarin Xinkeshu Granule Determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
