平阳霉素对人口腔癌细胞的抑制作用和对端粒酶活性的影响

目的:研究平阳霉素的抗癌机理,并初步探讨端粒酶作为化疗敏感性指标的可行性。方法:应用MTT法、双层琼脂培养法、倒置显微镜观察、HE染色观察、透射电镜观察、免疫组化法、TRAP-PCR-ELISA法研究平阳霉素对人舌癌Tca8113细胞的抑制作用和对端粒酶活性的影响。结果:平阳霉素对人舌癌Tca8113细胞有明显的抑制作用且呈浓度依赖性,对细胞大体形态和超微结构有明显的改变,下调c-myc、bac-2的表达,抑制端粒酶活性且有一定的时间依赖性(用416ng/ml平阳霉素处理Tca8113舌癌细胞后12h、24h、48h、72h、96h端粒酶活性分别为0.76±0.03,0.45±0.06,0.32±0.05,0.14±0.02,阴性)。结论:平阳霉素可以明显抑制Tca8113细胞的增殖活性及其转移复发倾向,还可作用于线粒体,在下调c-myc、bac-2的表达的同时抑制端粒酶活性。

Pingyangmycin inhibit cell activity and telomerase activity on oral carcinoma

Objective To study the anticancer mechanismthe of Pingyangmycin,and investigate the feasibility of making telomerase as sensitive index in chemotherapy. Methods Using the MTT assay,clonogenic assay, HE staining,transmission electron microscope observation,immunohistochemical staining and TRAP-PCR-ELISA in Tca8113 untreated and Tca8113 treated with Pingyangmycin. Results Cell activity of Tca8113 was significantly inhibited , and the effect was dependent on the concentration of Pingyangmycin. Morphology of cell was changed,the levels of c-myc?bac-2 was decreased compared with untreated Tca8113 cells. Telomerase activity was significantly and time-dependent inhibited when treated with Pingyangmycin. Conclusion The results indicated that Pingyangmycin can effectively inhibit telomerase and cell activity of Tca8113 cells. It can reduce the levels of c-myc?bac-2 at the same time.