Immunoglobulin VH gene expression in human B cell lines and tumors: biased VH gene expression in chronic lymphocytic leukemia |
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| Authors: | T Logtenberg M E Schutte G Inghirami J E Berman F H Gmelig-Meyling R A Insel D M Knowles F W Alt |
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| Institution: | Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, NY 10032. |
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| Abstract: | We have studied frequencies of VH gene utilization in a panel of monoclonal Epstein-Barr virus (EBV)-transformed B cell lines derived from human adult and fetal tissues as well as in monoclonal B cells obtained from fresh chronic lymphocytic leukemia (CLL) samples. The results show that IgM-secreting EBV cell lines from both fetal and adult tissues utilize VH genes from particular families roughly in proportion to estimated family size, suggesting that the repertoire of sigM-positive B cells in both fetal and adult organs is 'normalized' with respect to the V(H) gene family. In contrast, we find a highly biased pattern of VH gene expression in CLLs. The significance of these findings is discussed in the context of mechanisms that could be involved in normal B cell repertoire development and in the process of malignant transformation of precursors of CLL. |
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| Keywords: | human antibody repertoire human B cells CLL |
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