| PRL-3基因沉默对乳腺癌MCF-7细胞增殖和凋亡的影响 |
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| 引用本文： | 钟琰,吴爱国,纪术峰,沈三弟.PRL-3基因沉默对乳腺癌MCF-7细胞增殖和凋亡的影响[J].新医学,2011,42(6):370-374. |
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| 作者姓名： | 钟琰 吴爱国 纪术峰 沈三弟 |
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| 作者单位： | 南方医科大学珠江医院普外科,510282 |
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| 基金项目： | 广东省科技计划资助项目 |
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| 摘 要： | 目的：观察短发夹状小干扰RNA（shRNA）沉默PRL-3基因对人乳腺癌MCF-7细胞增殖、凋亡的影响。方法：构建PRL-3基因特异性shRNA表达载体,使用脂质体法将PRL-3-shRNA表达载体转染入MCF-7细胞。采用实时荧光定量PCR和蛋白免疫印迹法检测转染后MCF-7细胞PRL-3基因mRNA和蛋白的表达;运用MTT法检测转染后MCF-7细胞的增殖水平,流式细胞仪检测细胞凋亡情况。结果：酶切鉴定和测序分析证实PRL-3-shRNA表达载体成功构建。转染成功后,PRL-3-shRNA组PRL-3基因mRNA和蛋白表达水平明显降低。MTT结果显示MCF-7细胞转染PRL-3-shRNA后细胞增殖水平降低;流式细胞仪检测显示,PRL-3-shRNA转染后,MCF-7细胞凋亡率明显增高。结论：沉默PRL-3基因表达可抑制MCF-7细胞的增殖,促进其凋亡。
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| 关 键 词： | 乳腺癌 PRL-3 shRNA 基因治疗 细胞增殖 凋亡 |
Effects of PRL-3 gene on the proliferation and apoptosis of breast cancer MCF-7 cells |
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| ZHONG Yan,WU Ai-guo,JI Shu-feng,SHEN San-di.Effects of PRL-3 gene on the proliferation and apoptosis of breast cancer MCF-7 cells[J].New Chinese Medicine,2011,42(6):370-374. |
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| Authors: | ZHONG Yan WU Ai-guo JI Shu-feng SHEN San-di |
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| Institution: | ZHONG Yan,WU Ai-guo,JI Shu-feng,SHEN San-di.Department of General Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China |
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| Abstract: | Objective:To study the effects of short hairpin RNA(shRNA) targeting PRL-3 gene on the proliferation and apoptosis of breast cancer MCF-7 cells.Methods:The PRL-3 specific shRNA expression vector was constructed and confirmed by sequencing analysis.PRL-3-shRNA expression vector was transfected into MCF-7 cells via lipofectamineTM 2000,and the expression levels of PRL-3 mRNA and protein compared with those non-transfected cells were determined by real-time fluorescent quantitative PCR and western blotting.Flo... |
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| Keywords: | PRL-3 shRNA |
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