再生肝胞浆液对大鼠急性实验性肝功能衰竭的疗效研究

本文研究了同系和异种动物再生肝胞浆液对大鼠急性实验性肝衰的疗效,并初步探讨了作用机理,用10%氨基半乳糖腹腔注射引起大鼠暴发性肝功能衰竭、实验结果表明,与对照组和成年大鼠正常肝胞浆液治疗组比较,同系和异种动物(狗)再生肝胞浆液治疗组有显著提高病鼠的存活率的作用(P<0.01),~3H-胸苷参入的实验证明狗再生肝胞浆液治疗组肝DNA合成率为对照组的2.5倍,提示促进病损肝脏再生是其发挥治疗作用的重要机制,狗再生肝胞浆液治疗组于治疗后24小时及48小时,血清BSP潴留量和总胆红素浓度均显著低于对照组(P<0.05)。

EFFECT OF REGENERATING LIVER CYTOSOL ON EXPERIMENTAL ACUTE HEPATIC FAILURE IN RATS

This report describes the effect ofsyngeneic regenerating liver cytosol(SRLC) and xenogeneic (canine) regene-ratig liver cytosol (XRLC) on experi-mental acute hepatic failure in rats.Hepatic failure was induced with 10%D-galactosamine injected intraperitone-ally, and 48 hours later, the rats withhepatic failure were treated by intrape-ritoneal injection of saline (control)SRLC, XRLC and adult normal livercytosol (NLC) respectively. The resultshowed that survival of rats with hepaticfailure in SRLC and XRLC groups wasincreased signiflcantly, compared withcontrol and NLC groups (p<0.01). Therate of incorporation of ~3H-thymidineinto hepatic DNA of XRLC group was2.5 times as high as that of control,indicating that increase of the rate ofregeneration in the injured recipient liverplays an important role in improvingsurvival after hepaticf failure. Bothserum BSP retention and serum totalbilirubin were lower in XRLC groupthan those In control 24, 48 hours aftertreatment.