大小刺激量捻转补泻法对血虚证大鼠红细胞计数的影响

目的 :探讨大小刺激量捻转补泻法与补泻效应的关系。方法 :70只Wistar大鼠采用断尾放血法造成血虚证模型 ,随机分为 7个组 (A、B、C、D、E、F、G组 ) ,每组 10只 ,A、B组为对照组 ,C、D、E、F、G组分别采用不同捻转参数 (捻转幅度、频率和时间 )的捻转补泻手法针刺血虚证大鼠的“足三里”穴 ,观察对红细胞计数的影响。结果 :不同捻转幅度、捻转频率和捻转时间决定的捻转补泻法对血虚证大鼠模型的红细胞计数可产生不同影响。当捻转刺激量≤ 2圈 /次、60次 /min、2min操作时间时 ,呈现补法效应 ,当捻转刺激量大于此时 ,呈现泻法效应。结论 :以刺激量大小决定的捻转补泻法是客观存在的 ,并存在一个临界点 ,当刺激量小于这个临界点时产生补的效应 ,大于这个临界点时则产生泻的效应。

Effect of Acupuncture Stimulation Quantum on Red Blood Cell Count in Rats with Blood-deficiency Syndrome

Objective: To explore the relationship between the stimulation quantum of acupuncture and its effect on red blood cell (Rbc) count in blood-deficiency rats. Methods:Seventy Wistar rats were randomly and evenly assigned to A (control), B, C, D, E, F and G groups. Blood-deficiency model was established in all the animals by bleeding from the rat’s tail vessel (2 mL/time), once every other day, 3 times altogether. Rats of group B were just trussed up, and those of group C, D, E, F and G accepted acupuncture of “Zusanli”(ST 36). In group C, the needle was not manipulated after insertion. In group D, the needle was twirled for 0.5 min, half circle/time and 15 times/min. In group E, the needle was twirled for 1 min, 1 circle/time and 30 times/min; group F 2 min, 2 circles/time and 60 times/min; and group G 4 min, 4 circles/time and 120 times/min. Blood samples were collected from the caudal vessels for detecting Rbc count under microscope. Results: After bleeding, the number of Rbc in all the groups except group G all increased significantly in comparison with pre-bleeding (P<0.05～0.01). After bleeding, the counts of Rbc in group D, E and F were strikingly higher than that in group B (P<0.01), suggesting that small amplitude, low frequency and shorter duration of needling stimulation could promote production of Rbc. In group G, no significant change between pre- and post-bleeding, and no significant differences were found between group G and group B in the Rbc count, suggesting that bigger amplitude, higher frequency and longer duration of needling stimulation could suppress production of Rbc. It also displayed that when needling stimulation parameters were equal to or less than amplitude of 2 circles/time plus frequency of 60 times/minute and stimulating duration of 2 minutes, the red blood cell count increased considerably (P<0.01), which may be a critical point for generating favorable therapeutic effect; and if stronger acupuncture stimulation was given, the red blood cell count would not change. Conclusion: In the treatment of blood-deficiency syndrome, the needling stimulating quantum is rather important, and there is a critical point. When the quantum of stimulation is equal to or below the critical point, the acupuncture may produce a reinforcing effect, whereas, if it exceeds the critical point, the acupuncture may produce a reducing effect.