高脂饲料对OLETF大鼠糖代谢及血清磷脂脂肪酸组成的影响

目的:观察高脂饲料对自发性2型糖尿病模型OLETF大鼠糖代谢及血清磷脂脂肪酸组成的影响。方法:将20只14w雄性OLETF大鼠随机分为两组,分别以高脂及标准饲料喂饲10w,两种饲料中饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸占总脂肪酸的百分含量相似。大鼠第24w龄时进行口服糖耐量试验,同时测定大鼠的胰岛素水平和血清磷脂脂肪酸组成。结果:实验期间两组大鼠总进食量无统计学差异,但高脂饲料组大鼠体重明显高于标准饲料组(P<0.01);口服糖耐量试验和胰岛素水平测定结果表明,两组大鼠的糖代谢状态无统计学差异;血清磷脂脂肪酸组成中,除总n-6PUFA和18:3(n-3)的百分含量无统计学差异外,其他各脂肪酸组分在两组间的差异均具有统计学意义(P<0.01)。结论:在饲料脂肪酸组成保持可比的情况下,饲料脂肪量的增加可使OLETF大鼠的体重明显增加,而对糖代谢的影响不明显,同时对OLETF大鼠血清磷脂脂肪酸组成产生明显影响。

INFLUENCE OF HIGH-FAT DIET ON GLUCOSE METABOLISM AND FATTY ACID COMPOSITION OF SERUM PHOSPHOLIPID IN OLETF RATS

Objective: To investigate the influence of high-fat diet on glucose metabolism and fatty acid composition of serum phospholipid in OLETF rats, a model of spontaneous type 2 diabetes mellitus. Method: Twenty male OLETF rats of 14 w age were divided into 2 groups randomly. The dietary fat in two groups contained identical percentages of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids. Oral glucose tolerance test and measurement of insulin level were performed after 10 w treatment. The fatty acid composition of serum phospholipid in rats was observed as well. Results:The food intakes were comparable in 2 groups. The body weight gain in high-fat group was higher than standard group during the experiment. The result of oral glucose tolerance test and measurement of insulin level showed that the glucose metabolism in two groups had no significant difference. Only the percentage of total n-6 polyunsaturated fatty acids, 18:3(n-3) of serum phospholipid were comparable, percentages of other fatty acids were significantly different in two groups. Conclusion:With the comparable fatty acid composition, higher dietary fat lead to more body weight gain, but had no significant influence on glucose metabolism in OLETF rats. The amount of dietary fat had significant effect on fatty acids composition of serum phospholipid in OLETF rats.