绞股蓝、生山楂水提物对非酒精性脂肪性肝炎大鼠脂质代谢相关因子的调控作用研究

目的:探讨绞股蓝、生山楂水提物预防大鼠非酒精性脂肪性肝炎(Non-alcoholic Steatohepatitis,NASH)及其对肝组织SREBP-1c、PPARγ和PPARαmRNA表达水平的影响。方法:40只雄性SD大鼠,随机分为4组,每组10只。除正常对照组外,高脂饲料喂养10周建立大鼠非酒精性脂肪性肝炎模型。自造模即日起,正常对照组、模型组每天分别给予10mL/kg去离子水灌胃,中药组给予绞股蓝、生山楂水提物,西药组给予易善复混悬液灌胃。给药10周后,处死所有大鼠,观察各组大鼠肝指数,肝组织病理学改变及RT-PCR法检测肝组织SREBP-1c、PPARγ和PPARαmRNA表达水平。结果:与模型组比较,中药组体质量及肝指数明显降低(P<0.05),脂肪变、小叶内炎症病理评分明显下降(P<0.01),SREBP-1c mRNA的表达量显著降低(P<0.01),PPARαmRNA的表达量升高(P<0.05),PPARγmRNA表达量较模型组亦有所升高,但无显著统计学差异(P>0.05)。结论:绞股蓝、生山楂水提物可调控SREBP-1c、PPARγ和PPARα的基因表达,对非酒精性脂肪性肝炎有预防作用。

Research on Adjustment of Lipid Metabolism Factors by Extracts of Jiaogulan and Shengshanzha Toward Non-alcoholic Steatohepatitis Rats

Objective:To investigate the effect of the extracts of Jiaogulan and Shengshanzha toward non-alcoholic steatohepatitis rats.Method:Forty male SD rats were randomly divided into four groups,each group had ten.In addition to the control group,others were fed with high fat diet for 10 weeks to reproduce NASH rats.Since the modeling day,the control group and the model group were given deionized water 10mL/kg daily.The Chinese herb group were given the extracts of Jiaogulan and Shengshanzha,and the western medicine group were given Essentiale.After ten weeks,all rats were killed,then we observed the liver index,pathological changes and the the expressions of SREBP-1c,PPARγ and PPARαmRNA of liver.Results:Compared with modle group,the weight and liver index of Chinese herb group decreased significantly(P<0.05),the steatosis,lobular inflammation and the expression of SREBP-1c mRNA decreased significantly(P<0.01),the expression of PPARα mRNA increased significantly(P<0.05),and the expression of PPARγmRNA had the tend to increase(P>0.05).Conclusion:The extracts of Jiaogulan and Shengshanzha can regulate the expressions of SREBP-1c,PPARγ and PPARα mRNA,those show that the extracts can play the efficacy of preventing NASH.