N-Hydroxylation of 2-aminofluorene in the guinea pig and by guinea pig liver microsomes in vitro

Summary N-hydroxy-2-aminofluorene was found in the urine of guinea pigs intraperitoneally injected with 2-aminofluorene. The hydroxylamine was oxidized to the nitroso analogue and this was identified and determined in the carbon tetrachloride extract by its characteristic UV absorption, by thin-layer chromatography, and by the formation of a diazo compound in the reaction with nitrous acid. Only a small fraction of the 2-aminofluorene injected appeared in the urine as N-hydroxy derivative.Guinea pig liver microsomes were observed to N-hydroxylate 2-aminofluorene rather rapidly, the reaction proceeding at least as rapidly as the N-hydroxylation of aniline.The results of this paper were presented at meetings of the Deutsche Pharmakologische Gesellschaft in Mainz, April 26 to 28, 1965 (Kampffmeyer and Kiese) and Göttingen, September 27 to 30, 1965 (von Jagow, Kiese, Renner, and Wiedemann).